Construct: ORF ccsbBroad304_03738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009785.1_s304c1, BRDN0000410687
- Derived from:
- ccsbBroadEn_03738
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM9B (56674)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03738
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_020644.3 | 100% | 100% | |
| 2 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_001286094.1 | 62.6% | 62.6% | 0_1ins222 |
| 3 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_001286095.1 | 62.6% | 62.6% | 0_1ins222 |
| 4 | mouse | 56786 | Tmem9b | TMEM9 domain family, member B | NM_020050.1 | 88.9% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccctgtgg ggaggccttc ttcggcttgg ctccttgctc agcctgtcgt 121 gcctggcgct ttccgtgctg ctgctggcgc agctgtcaga cgccgccaag aatttcgagg 181 atgtcagatg taaatgtatc tgccctccct ataaagaaaa ttctgggcat atttataata 241 agaacatatc tcagaaagat tgtgattgcc ttcatgttgt ggagcccatg cctgtgcggg 301 ggcctgatgt agaagcatac tgtctacgct gtgaatgcaa atatgaagaa agaagctctg 361 tcacaatcaa ggttaccatt ataatttatc tctccatttt gggccttcta cttctgtaca 421 tggtatatct tactctggtt gagcccatac tgaagaggcg cctctttgga catgcacagt 481 tgatacagag tgatgatgat attggggatc accagccttt tgcaaatgca cacgatgtgc 541 tagcccgctc ccgcagtcga gccaacgtgc tgaacaaggt agaatatgca cagcagcgct 601 ggaagcttca agtccaagag cagcgaaagt ctgtctttga ccggcatgtt gtcctcagct 661 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 721 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 781 ggatta