Construct: ORF ccsbBroad304_04284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006548.1_s304c1, BRDN0000405947
- Derived from:
- ccsbBroadEn_04284
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMAC (83640)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04284
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83640 | RAMAC | RNA guanine-7 methyltransfe... | NM_031452.4 | 100% | 100% | |
| 2 | mouse | 67148 | Fam103a1 | family with sequence simila... | NM_025997.2 | 84.6% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tgacactgcc gaagctgttc caaagtttga agagatgttt gctagtagat 121 tcacagaaaa tgacaaggag tatcaggaat acctgaaacg ccctcctgag tctcctccaa 181 ttgttgagga atggaatagc agagctggtg ggaaccaaag aaacagaggc aatcggttgc 241 aagacaacag acagttcaga ggcagggaca acagatgggg gtggccaagt gacaatcgat 301 ccaatcagtg gcatggacga tcctggggta acaactaccc gcaacacaga caagaacctt 361 actatcccca gcaatatgga cattatggtt acaaccagcg gcctccttac ggttactact 421 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta