Construct: ORF ccsbBroad304_04345

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008680.1_s304c1, BRDN0000460256
Derived from:
ccsbBroadEn_04345
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MED10 (84246)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04345
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84246 MED10 mediator complex subunit 10 NM_032286.3 100% 100%
2 mouse 28077 Med10 mediator complex subunit 10 NM_138596.2 89.6% 99.2% (many diffs)
3 mouse 28077 Med10 mediator complex subunit 10 XM_011244527.2 73.7% 81.7% (many diffs)
4 mouse 28077 Med10 mediator complex subunit 10 XM_006517275.3 55.2% 57.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
471
ORF length:
405
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggagaagttt gaccacctag aggagcacct ggagaagttc gtggagaaca
121ttcggcagct cggcatcatc gtcagtgact tccagcccag cagccaggcc gggctcaacc
181aaaagctgaa ttttattgtt actggcttac aggatattga caagtgcaga cagcagcttc
241atgatattac tgtaccgtta gaagtttttg aatatataga tcaaggtcga aatccccagc
301tctacaccaa agagtgcctg gagagggctc tagctaaaaa tgagcaagtt aaaggcaaga
361tcgacaccat gaagaaattt aaaagcctgt tgattcaaga actttctaaa gtatttccgg
421aagacatggc taagtatcga agcatccggg gggaggatca cccgccttct tacccaactt
481tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
541agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)