Construct: ORF ccsbBroad304_04868

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014492.1_s304c1, BRDN0000402423
Derived from:
ccsbBroadEn_04868
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
M1AP (130951)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04868
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 130951 M1AP meiosis 1 associated protein NM_001321739.2 100% 100%
2 human 130951 M1AP meiosis 1 associated protein NM_138804.4 100% 100%
3 human 130951 M1AP meiosis 1 associated protein XM_011532550.2 100% 100%
4 human 130951 M1AP meiosis 1 associated protein XM_011532551.2 100% 100%
5 human 130951 M1AP meiosis 1 associated protein NM_001281296.1 99.2% 99.2% 1419_1420insAAGCATCCCTGC
6 human 130951 M1AP meiosis 1 associated protein XM_006711946.3 93.3% 93.3% 1_114del
7 human 130951 M1AP meiosis 1 associated protein XM_011532548.2 92.6% 92.6% 1_114del;1533_1534insAAGCATCCCTGC
8 human 130951 M1AP meiosis 1 associated protein XM_011532549.2 89.7% 89.7% 1_114del;1046_1047ins60
9 human 130951 M1AP meiosis 1 associated protein XM_024452711.1 86.2% 86.2% 1073_1074ins207;1212_1213insAAGCATCCCTGC
10 human 130951 M1AP meiosis 1 associated protein XM_011532552.2 83% 82.9% 1_114del;709_710ins174
11 human 130951 M1AP meiosis 1 associated protein XM_011532553.2 81.1% 81.1% 1_114del;1187_1188ins207
12 human 130951 M1AP meiosis 1 associated protein NM_001281295.2 68.3% 67.9% (many diffs)
13 human 130951 M1AP meiosis 1 associated protein XM_011532554.2 53.4% 52.2% (many diffs)
14 human 130951 M1AP meiosis 1 associated protein XM_005264152.3 52.6% 51.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1656
ORF length:
1590
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca tcctgggcga actactggta aagggccctc tactcacact cagattgacc
121agcaacctcc acggcttctc attgtgcaca ttgctctacc gtcctgggct gacatctgca
181ccaacctctg tgaggctctg cagaacttct tctctctagc ctgcagcttg atgggcccca
241gccgcatgtc cctgttcagt ttatacatgg tacaagatca gcatgagtgc atcctccctt
301ttgtgcaagt gaaagggaac tttgctaggt tgcagacctg catctcagaa ctccgcatgt
361tacagagaga agggtgtttc agatcacaag gtgcttctct gcggctggca gtagaggatg
421ggctccagca attcaaacaa tacagcagac atgtgaccac aagggcagct ctgacctata
481cctccctgga gattactatt ctgacttctc agcctggaaa agaggtggtc aaacagttgg
541aggaagggtt gaaagataca gacctagcca gagtcaggag gtttcaggtc gttgaggtca
601caaagggaat cctagagcac gtggactcag cgtctcctgt tgaggatacc agcaatgatg
661agagttctat tctgggaact gacattgacc ttcagactat agacaatgat atcgtcagca
721tggagatttt cttcaaagcc tggctacata acagtggaac agaccaagaa caaatccatc
781ttcttctttc ttcacagtgt ttcagcaaca tttccagacc cagagataat ccaatgtgtc
841tgaaatgtga tctccaagag cgactgctct gcccatccct actcgctggc acagctgacg
901gctccttgag aatggatgac cctaaaggag acttcatcac actctaccag atggcttccc
961agtcatcggc ctctcattac aagctccaag tgatcaaggc tttaaaatct agcgggctct
1021gcgagtcatt gacatatgga ctcccgttca tcctcagacc tacaagctgt tggcagctgg
1081actgggatga gctggagaca aatcagcaac atttccatgc tttgtgtcac agcctgctga
1141aaagggaatg gctgctgtta gccaaggggg aaccaccggg cccaggacac agccagagaa
1201ttcctgccag caccttctat gtgatcatgc cgtcacactc cctcacactg ctggtaaagg
1261cggtggccac gcgggaactg atgctgccca gcaccttccc cctgctacct gaggacccac
1321atgatgatag ccttaagaat gtggagagca tgctggacag cctggagctg gagcccacct
1381acaacccctt gcatgttcaa agccacctgt actcacacct gagcagcatc tatgccaagc
1441ctcaggggcg gctccaccca cactgggaga gccgagctcc gagaaagcat ccctgcaaga
1501ctgggcagtt gcagaccaac cgagctcgag ctactgtggc ccccctgcct atgactcctg
1561tcccaggcag agcctccaag atgccagcag ccagcaaatc ttcctcagat gccttcttcc
1621tgccttcaga gtgggagaag gatccctcaa ggccctaccc aactttcttg tacaaagtgg
1681ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1741ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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