Construct: ORF ccsbBroad304_04946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007317.1_s304c1, BRDN0000461097
- Derived from:
- ccsbBroadEn_04946
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MACROD2 (140733)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04946
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | NM_001033087.2 | 100% | 100% | |
| 2 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | NM_001351664.2 | 88.7% | 88.7% | 281_361del |
| 3 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | XM_017027675.1 | 86.2% | 86.2% | 66_167del |
| 4 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | XM_024451834.1 | 77.7% | 77.7% | 66_167del;383_463del |
| 5 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | XM_017027676.1 | 76.9% | 76.9% | 66_167del;627_628ins69 |
| 6 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | NM_001351661.2 | 47.5% | 47.5% | 1_705del |
| 7 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | NM_001351663.2 | 47.3% | 47.3% | 1_705del;1298_1299insAGC |
| 8 | human | 140733 | MACROD2 | mono-ADP ribosylhydrolase 2 | NM_080676.6 | 42.4% | 42.4% | 1_705del;1230_1231ins69 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 708
- ORF length:
- 639
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatgagttt ttctccgtag acgataataa tgaagaagaa gaggatgttg 121 aaatgaaaga agattcagat gagaacggtc cagaggagaa gcaaagtgtg gaagaaatgg 181 aagagcagag ccaagatgca gatggtgtca acactgtcac tgtgcccggc cctgcttcag 241 aagaggcagt tgaagactgt aaagatgaag attttgcaaa ggatgaaaat attacaaaag 301 gcggtgaagt gacagatcat tctgtgcgtg accaagatca tcccgatgga caagagaatg 361 attcaacgaa gaatgaaata aaaattgaaa cagaatcgca gagctcatat atggaaacag 421 aagaactttc atcaaaccaa gaagatgccg tgattgtgga gcaaccagaa gtgattccat 481 taacagagga ccaagaagaa aaagaaggtg aaaaagctcc aggcgaggac acacctagga 541 tgcctgggaa aagtgaaggc tccagtgacc tagaaaatac tccaggtcct gatgttgaaa 601 tgaatagtca ggttgacaag gtaaatgacc caacagagag tcaacaagaa gatcaactaa 661 tagcaggggc acaagatgaa gcgaaggaac aaagaaatgg aactaaattg ccaactttct 721 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 781 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta