Construct: ORF ccsbBroad304_05065

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009134.1_s304c1, BRDN0000407795
Derived from:
ccsbBroadEn_05065
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPICE1 (152185)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05065
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 152185 SPICE1 spindle and centriole assoc... NM_001331078.2 100% 100%
2 human 152185 SPICE1 spindle and centriole assoc... NM_144718.4 100% 100%
3 human 152185 SPICE1 spindle and centriole assoc... NM_001331079.1 98.3% 98.3% 1_42del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2631
ORF length:
2565
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc atttgtcaga gtgaaccgct gtggtccccg agttggtgta agaaagacac
121cgaaagtaaa gaagaagaaa acttcagtga aacaagaatg ggataatacc gtgactgatc
181taaccgttca tcgggcaact cccgaagatc tggtacgccg tcatgaaata cacaaatcga
241agaatagagc attagtacac tgggaactcc aagaaaaagc tttgaagaga aaatggagga
301agcagaaacc agaaacttta aatcttgaga aaagaagatt gtctatcatg aaggagattc
361tttctgatca ataccagatg caagatgtgt tggagaaatc tgatcatcta atagctgcag
421caaaagagct gtttcctcgt aggcgcacag ggtttccaaa tgtaacagtg gctcctgatt
481cctctcaggg tcccattgtg gtaaatcaag accctatcac ccaatctatc tttaatgagt
541ctgtcataga acctcaggct cttaatgatg tagatggtga agaagaagga actgttaata
601gccagtcagg agaaagtgag aatgagaatg agttggataa ctctctaaac tctcagtcta
661acacgaatac agacaggttt ctccaacaac taacagaaga gaattttgag ttaattagta
721agttgtggac tgacattcag cagaaaatag caacccagtc acaaataact cctccaggaa
781cgccatcatc tgctctttca tcaggggagc aaagagctgc tctgaatgct accaatgctg
841tcaagagact ccaaaccagg cttcagcctg aagaatctac tgagactcta gactcaagct
901acgttgtggg acacgtgctg aactcaagga agcaaaaaca gctgttaaat aaagtgaaaa
961ggaaaccgaa tttgcatgct ctttccaagc cgaagaaaaa catatcatca ggtagcacaa
1021cctctgcaga cttaccaaat aggactaatt ccaacctgga tgtcctcaaa cacatgatac
1081atgaagtgga acatgaaatg gaagaatatg agcggtggac aggtcgcgag gtcaagggtc
1141tgcagagcag tcagggtctt acaggcttca ctttgtcgct ggtgagctcc ctctgtcgcc
1201tggttcggta ccttaaagag agtgagatcc agctacgtaa agaagtagag acaaggcaac
1261aactggaaca agtattaggt gatcatcgag agctcattga tgctctgaca gctgaaattc
1321ttcgtcttag agaagaaaac gctgctacac aggcaagact tcagcagtac atggtcacaa
1381cagatgagca actgatatca ctcacacatg ctattaagaa ctgtcctgtg ataaataaca
1441gacaagaaat tcaggcatca gaaagcggag ccacaggtag aagagttatg gacagtccag
1501agcgtccagt tgtaaatgcc aatgtctcag tgccattgat gttcagagag gaagtggctg
1561aattcccaca ggaagagttg cccgttaaac tgtctcaggt gccagaccct ccagataaca
1621tgaatctggc caagaatttt ccagcacata tttttgagcc agctgtgttg ttaacaccac
1681ccaggcagaa gagcaactta aaattctctc ctcttcagga cgtattgaga aggactgttc
1741aaactcgtcc tgctccacga cttcctccaa ctgtggaaat aattgagaag gaacaaaatt
1801gggaagagaa gaccttacct attgatacag acattcagaa ttcaagtgaa gagaatcgtc
1861tcttcactca gagatggaga gtctctcaca tgggagaaga tttggagaac aaaactcagg
1921ctccttttgt taacctctca cagcccctct gcaattccca ttccaacact caacagtcaa
1981gaagccccac attctcagaa gagctcccag tactgggaga tgggcagcag ctgagaacaa
2041atgagtcatt aatacaaaga aaggacataa tgacacgaat tgctgatttg acattgcaga
2101attcagctat caaggcacat atgaataata ttattgagcc cagaggagag caaggggatg
2161gactccggga gttgaacaaa caagaaagtg caagtgacat gacttctact tttccagtag
2221cacagtctct aacaccaggt agtatggagg aacggattgc agaattgaat cgacaaagta
2281tggaggctcg tggaaaacta ctgcagttga tagagcagca gaaacttgtt ggtttgaatc
2341tttctccacc aatgtcacct gttcagttac ctctcagagc atggactgaa ggagcaaaga
2401ggacaattga ggtatctatt ccaggagcag aagccccaga aagctcaaaa tgtagtactg
2461tctctcccgt cagcgggata aatacaagaa gatcttccgg ggctactggt aattcttgtt
2521ctccactaaa tgccacctca ggaagtggga gattcacacc tcttaatcca agagcaaaga
2581ttgagaaaca gaatgaagaa ggctggtttg ctctttctac ccatgtatca tacccaactt
2641tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2701agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)