Construct: ORF ccsbBroad304_05214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011808.1_s304c1, BRDN0000408398
- Derived from:
- ccsbBroadEn_05214
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VMA21 (203547)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05214
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203547 | VMA21 | vacuolar ATPase assembly fa... | NM_001017980.3 | 100% | 100% | |
| 2 | human | 203547 | VMA21 | vacuolar ATPase assembly fa... | NM_001363810.1 | 62% | 55.6% | (many diffs) |
| 3 | mouse | 67048 | Vma21 | VMA21 vacuolar H+-ATPase ho... | NM_001081356.3 | 86.7% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 372
- ORF length:
- 303
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcgcccg gataaggcgg cgctgaacgc actgcagcct cctgagttca 121 gaaatgaaag ctcattagca tctacactga agacgctcct gttcttcaca gctttaatga 181 tcactgttcc tattgggtta tatttcacaa ctaaatctta catatttgaa ggcgcccttg 241 ggatgtccaa tagggacagc tatttttacg ctgctattgt tgcagtggtc gccgtccatg 301 tggtgctggc cctctttgtg tatgtggcct ggaatgaagg ctcacgacag tggcgtgaag 361 gcaaacagga tttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 421 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 481 caatcaacct ctggatta