Construct: ORF ccsbBroad304_05227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002586.1_s304c1, BRDN0000462423
- Derived from:
- ccsbBroadEn_05227
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF804B (219578)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05227
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219578 | ZNF804B | zinc finger protein 804B | NM_181646.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4116
- ORF length:
- 4047
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttgttac ctggtcatca gttcgagaca tctcagcaat gggcactacc 121 ggggcattaa aggagtcttc aggggacccc tgtgcaagaa cggatctccc tctccggatt 181 ttgcagaaaa gaagtccaca gcaaaggccc tggaagatgt aaaggcaaac ttttactgtg 241 aattatgtga caagcagtat cacaaacacc aggagtttga caatcatatt aattcttatg 301 accatgctca taagcagaga ctgaaagaat taaagcaacg ggaatttgct cgaaatgtag 361 cttctaagtc atggaaagat gagaaaaaac aagaaaaagc acttaaacga cttcatcagc 421 tggctgagtt aaggcagcaa tctgaatgtg tttctggaaa tggaccagca tacaaagccc 481 ccagggtagc catagaaaag caactccagc aaggaatttt ccccattaag aatggcagaa 541 aggtatcatg catgaagagt gctcttctcc ttaaaggaaa aaatctcccc agaatcatat 601 ccgataaaca gcggtccacc atgccaaatc gacaccaatt acaatcagac aggcgttgtt 661 tgtttggaaa tcaggtactg caaacatctt cagatctcag caatgcaaat cacagaacag 721 gagtatcatt tactttttcc aaaaaagtgc acctaaaatt agaatcttca gcatcagttt 781 tcagtgagaa cacagaagaa acccatgatt gtaacaagtc acccatttat aaaacaaaac 841 aaactgcaga taagtgcaag tgctgcaggt ttgcaaataa agatacacac cttaccaagg 901 aaaaagaggt aaatatctca ccaagccatc tggaaagtgt tttacacaat accatctcca 961 taaactctaa aattttgcaa gacaaacacg actctattga tgagacacta gaagattcaa 1021 ttggcattca tgcttcattc tctaaatcta acattcatct ttcagatgta gattttactc 1081 ctaccagcag agaaaaagaa actagaaata cattgaagaa cactttagaa aattgtgtta 1141 atcacccatg ccaagcaaat gcttccttca gcccaccaaa catttacaac catagtgatg 1201 ccaggatatc tgaatgcctg gatgagtttt catcactgga gccaagtgaa caaaagagta 1261 cagtgcatct gaatccaaat tccagaatag agaacagaga aaaatcttta gataaaacag 1321 aaagagttag caaaaatgtt caaagacttg taaaagaagc atgtacccat aatgtggcat 1381 ctaaaccact accttttctc cacgttcaaa gcaaggatgg ccacaccact cttcaatggc 1441 ctacggaact tctgctcttt acaaaaacag aaccctgtat ctcttatggc tgcaacccac 1501 tgtattttga ttttaagctt tctcggaaca caaaggaaga ccacaatcta gaggacttaa 1561 aaacagaatt gggtaagaag cccttggaat tgaagactaa aagagagagc caagtctcag 1621 gtttaactga agaccaacaa aaattgatcc aagaagatta tcaatatccg aaaccaaaga 1681 cgatgatagc taatccggat tgggaaaaat tccagaggaa atataatttg gactacagtg 1741 attctgagcc aaataagagt gaatatactt tcagtgcaaa tgatttggaa atgaaaaatc 1801 ctaaagtgcc tctttacctc aacacatctc taaaggattg tgctggaaag aataatagta 1861 gtgagaacaa acttaaggaa gcttcaaggg cccattggca aggctgcaga aaggcagttc 1921 taaatgatat agatgaggac ctatcttttc cttcctacat ctctaggttt aaaaagcata 1981 aattgattcc ctgcagtcct catttggaat ttgaagatga aagacaattc aactgcaagt 2041 ccagtccttg tacagtaggg ggtcacagtg accatgggaa agacttcagt gtaattttga 2101 agagtaacca catcagcatg accagcaagg tttccggatg tggaaaccaa agatacaaga 2161 gatactctcc acagtcatgt ttgagtagat attcttcctc tttggacaca tcccctagca 2221 gcatgtctag cttgagaagt acttgttcaa gtcatagatt caatggtaat agcagaggta 2281 atttgctctg cttccataaa agagaacacc actcagttga aaggcacaaa cggaaatgtc 2341 taaagcacaa ctgcttctac ttgtctgatg atataacaaa gagcagccaa atgcagtctg 2401 aaccacagaa agagaggaac tgcaaattgt gggaatcatt taaaaatgaa aaatactcaa 2461 aacgtagata ttgtcactgc agagaaagac aaaaactggg caaaaatcaa caacaatttt 2521 cagggctaaa atctacgaga atcatctatt gtgattctaa ctcacagatt tcctgtactg 2581 gaagcagtaa aaaaccacct aattgccagg gaactcagca cgacagattg gactcttact 2641 caatagagaa aatgtattac ttgaataaaa gcaagagaaa tcaagagtct ttgggcagcc 2701 ctcacatttg tgatctggga aaagtcaggc ccatgaagtg taactccggg aatatcagct 2761 gccttctaaa gaactgttcc agtggccctt cagaaaccac agaatcaaac actgcagaag 2821 gagagaggac ccctctaaca gcaaaaatcc ttttagaaag agtacaagcc aagaaatgtc 2881 aagaacaatc aagtaatgtt gagatctctt caaacagttg taaaagtgaa ttagaggctc 2941 cttcgcaagt cccatgcaca attcaacttg caccatcagg ctgtaacaga caagcattgc 3001 ctttgtctga aaaaatacag tatgcaagtg agagcagaaa tgatcaagac agtgcaattc 3061 caaggactac ggagaaagac aaaagcaaaa gttcacacac aaataatttt acaattttag 3121 cagacactga ttgtgataac catctttcta aaggtataat tcacctagta acagagtctc 3181 agtcactaaa cataaaaagg gatgcaacaa caaaagaaca atcaaaacct ttaattagtg 3241 aaatccaacc ttttattcaa agctgtgacc cagtaccaaa tgaattccct ggtgcttttc 3301 cgtctaataa atatactggt gtgactgatt caacagagac ccaagaagac caaataaatc 3361 tagacttaca ggatgtaagc atgcatataa atcatgtaga gggaaatata aactcttact 3421 atgacagaac tatgcagaaa cctgacaaag tcgaagacgg attagaaatg tgtcataaat 3481 ctatctctcc ccctttaatt caacagccca taacattttc tcctgacgaa atagataaat 3541 ataagatcct acagctacaa gcccagcagc atatgcagaa gcaactccta tcaaagcatc 3601 ttcgagtttt gcctgctgca gggcctactg ccttctctcc ggcctcaacc gtacagacag 3661 ttccagttca ccagcacact tctatcacca ccatccacca cacgttcctg cagcattttg 3721 ctgtttctgc ttccttaagt tctcatagca gtcacctccc tattgctcat ctacatcctc 3781 tttcacaggc acatttcagt cctatttcat tttcgactct gactccaacc attatccctg 3841 cacaccccac tttcttagca ggtcatcccc tgcatttagt agctgctacc cccttccacc 3901 catctcacat aacacttcag cctctgcccc ctacagcatt tattcctaca ttgtttggtc 3961 ctcacttaaa tccagccaca acttctatca tccacttgaa tcctttaatc caaccagtat 4021 tccaaggtca agatttttgc catcattctt gctctagcca gatgcaacag ctaaatgaag 4081 tgaaagaggc cttaaatgtg tccacacact tgaacttgcc aactttcttg tacaaagtgg 4141 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 4201 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta