Construct: ORF ccsbBroad304_05262
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006068.1_s304c1, BRDN0000404763
- Derived from:
- ccsbBroadEn_05262
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AC1 (221613)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05262
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221613 | H2AC1 | H2A clustered histone 1 | NM_170745.3 | 100% | 100% | |
| 2 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 80.4% | 93.8% | (many diffs) |
| 3 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 79.4% | 93.1% | (many diffs) |
| 4 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 77.7% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggacgaggg aagcagggag gaaaagcacg cgccaagtct aagtctcgct 121 cttctagagc gggtttgcag tttcccgtag gccggatcca tcgtctgctt cgtaagggaa 181 actatgcaga gcggataggg gcaggcgcac cagtgtattt ggcggcagtg ttagagtatc 241 tcacagcaga aatccttgag ctggcaggca atgcgtctcg cgataacaaa aaaactcgca 301 ttattccccg ccacctgcag ctagcgatcc gcaatgatga ggaactcaat aagcttttgg 361 gcggcgtgac cattgcccag ggcggagtcc tgcctaacat tcaggcagtg ctgctgccca 421 agaagactga gagtcaccac cataaagccc aaagcaagta cccaactttc ttgtacaaag 481 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 541 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta