Construct: ORF ccsbBroad304_05278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008180.1_s304c1, BRDN0000411432
- Derived from:
- ccsbBroadEn_05278
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFB119 (245932)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05278
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 245932 | DEFB119 | defensin beta 119 | NM_153289.4 | 100% | 100% | |
| 2 | human | 245932 | DEFB119 | defensin beta 119 | NR_073153.1 | 45.4% | 1_166del;225_264del;459_555del | |
| 3 | human | 245932 | DEFB119 | defensin beta 119 | NR_073151.1 | 42.5% | 1_166del;225_301del;496_592del | |
| 4 | human | 245932 | DEFB119 | defensin beta 119 | NR_073152.1 | 37.6% | (many diffs) | |
| 5 | human | 245932 | DEFB119 | defensin beta 119 | NR_126440.2 | 36.9% | 1_107del;165_166ins88;272_356del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 318
- ORF length:
- 252
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa acttctttac ctgtttcttg ccatccttct ggccatagaa gaaccagtga 121 tatcaggcaa acgccacatc cttcgatgca tgggtaacag tggaatttgt agggcctctt 181 gcaaaaagaa cgaacagccc tacctctatt gcagaaattg tcagtcctgc tgcctccagt 241 cctacatgag gataagcatt tctggcaaag aggaaaatac cgactggtct tatgagaagc 301 agtggccaag actaccttgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 361 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 421 agtcgacaat caacctctgg atta