Construct: ORF ccsbBroad304_05357
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017441.1_s304c1, BRDN0000411645
- Derived from:
- ccsbBroadEn_05357
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATC (283459)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05357
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283459 | GATC | glutamyl-tRNA amidotransfer... | NM_176818.3 | 100% | 100% | |
| 2 | human | 283459 | GATC | glutamyl-tRNA amidotransfer... | NR_033684.2 | 9.4% | 1_37del;288_385del;544_4336del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggtcgcgg ttggtgtggc tgggccttcg ggcccctctg ggcgggcgcc 121 agggcttcac ctccaaggcg gatcctcagg gcagtggccg gatcacggct gcggtgatcg 181 agcacctgga gcgtctagcg cttgtggact tcggcagccg cgaggcagtg gcgcgactgg 241 agaaagctat cgccttcgcc gaccggctac gcgccgtgga cacagacggg gtggagccca 301 tggaatcggt cctggaggac agatgtctat acctgagatc cgacaatgtg gtagaaggca 361 actgtgctga tgaattacta caaaactccc atcgcgtcgt ggaggagtac tttgtggccc 421 ccccaggtaa tatctctttg ccaaagctgg atgaacaaga gccattccca cacagcttgc 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta