Construct: ORF ccsbBroad304_05588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009095.1_s304c1, BRDN0000402017
- Derived from:
- ccsbBroadEn_05588
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LDLRAD1 (388633)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05588
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | NM_001010978.4 | 100% | 100% | |
| 2 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | XM_011541446.1 | 99.5% | 99.5% | 71_72insAGC |
| 3 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | NM_001276393.1 | 79% | 79% | 71_72ins129 |
| 4 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | NM_001276392.1 | 74.6% | 74.6% | (many diffs) |
| 5 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | NM_001276394.2 | 56.5% | 56% | 73_74ins267 |
| 6 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | NM_001276395.2 | 48.7% | 19.3% | 71_72ins160;300_301ins155 |
| 7 | human | 388633 | LDLRAD1 | low density lipoprotein rec... | XM_017001264.1 | 48.7% | 19.3% | 71_72ins160;300_301ins155 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 684
- ORF length:
- 615
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacaaggtc ttcccccagg gagagaatgg ctacactgct gctgaatcca 121 aagcccaccc tggaggggaa gcaggcggcg gccacctctg ctgctcacgt cgcggggcct 181 gcctctctgc ctctctgctg ctcctcctgg caactgtggc ggccctcatc gccttggtca 241 ccattcttgg actcccatca tgcaccccag gagcccaagc ttgtataaca ctgacaaaca 301 ggacaggctt cttgtgccat gaccagagga gctgcattcc agccagtggg gtctgtgatg 361 gcgttcgcac ctgtacccac ggcgaggacg aggatgagag cttgtgccga gatgtgcccc 421 agagcctccc ccacttcctt gtggcccact gtggagaccc ggcctcctgg atctactcag 481 accaaaaatg tgatggcact aacaactgcg gggactgttc agatgaactg agcccagtaa 541 ctgtgtgccc accctgcggc cctgggtggt ggcgctgtcc ttcaaccttc ttcaagtact 601 gcgactgtat accgaggcat ctctgccgcg accatgtaca gcactgctcc gactggtccg 661 atgagtatgc ctgtcccgga cccttgccaa ctttcttgta caaagtggtt ggtaagccta 721 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 781 gcgttaagtc gacaatcaac ctctggatta