Construct: ORF ccsbBroad304_05820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009535.1_s304c1, BRDN0000406269
- Derived from:
- ccsbBroadEn_05820
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A6 (293)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05820
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 293 | SLC25A6 | solute carrier family 25 me... | NM_001636.4 | 99.7% | 99.6% | 408C>T;725C>T |
| 2 | human | 292 | SLC25A5 | solute carrier family 25 me... | NM_001152.5 | 78.6% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ggaacaggcc atctccttcg ccaaagactt cttggccgga ggcatcgccg 121 ccgccatctc caagacggcc gtggctccga tcgagcgggt caagctgctg ctgcaggtcc 181 agcacgccag caagcagatc gccgccgaca agcagtacaa gggcatcgtg gactgcattg 241 tccgcatccc caaggagcag ggcgtgctgt ccttctggag gggcaacctt gccaacgtca 301 ttcgctactt ccccactcaa gccctcaact tcgccttcaa ggataagtac aagcagatct 361 tcctgggggg cgtggacaag cacacgcagt tctggaggta ctttgcgggc aacctggcct 421 ccggcggtgc ggccggcgcg acctccctct gcttcgtgta cccgctggat tttgccagaa 481 cccgcctggc agcggacgtg ggaaagtcag gcacagagcg cgagttccga ggcctgggag 541 actgcctggt gaagatcacc aagtccgacg gcatccgggg cctgtaccag ggcttcagtg 601 tctccgtgca gggcatcatc atctaccggg cggcctactt cggcgtgtac gatacggcca 661 agggcatgct ccccgacccc aagaacacgc acatcgtggt gagctggatg atcgcgcaga 721 ccgtgacggc cgtggccggc gtggtgtcct accccttcga cacggtgcgg cggcgcatga 781 tgatgcagtt cgggcgcaaa ggagctgaca tcatgtacac gggcaccgtc gactgttgga 841 ggaagatctt cagagatgag gggggcaagg ccttcttcaa gggtgcgtgg tccaacgtcc 901 tgcggggcat ggggggcgcc ttcgtgctgg tcctgtacga cgagctcaag aaggtgatct 961 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1021 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1081 ggatta