Construct: ORF ccsbBroad304_05849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011804.1_s304c1, BRDN0000407103
- Derived from:
- ccsbBroadEn_05849
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL1 (400)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05849
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400 | ARL1 | ADP ribosylation factor lik... | NM_001177.6 | 99.8% | 99.4% | 540A>C |
| 2 | human | 400 | ARL1 | ADP ribosylation factor lik... | NM_001301068.1 | 73.4% | 72.9% | 0_1ins141;2_4delTGG;402A>C |
| 3 | mouse | 104303 | Arl1 | ADP-ribosylation factor-like 1 | NM_025859.3 | 91.7% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tggctttttc tcaagtatat tttccagtct gtttggaact cgggaaatga 121 gaattttaat tttgggatta gatggagcag gaaaaaccac aattttgtac agattacaag 181 tgggagaagt tgttactact atacctacca ttggatttaa tgtagagacg gtgacgtaca 241 aaaaccttaa attccaagtc tgggatttag gaggacagac aagtatcagg ccatactgga 301 gatgttacta ttcaaacaca gatgcagtca tttatgtagt agacagttgt gaccgagacc 361 gaattggcat ttccaaatca gagttagttg ccatgttgga ggaagaagag ctgagaaaag 421 ccattttagt ggtgtttgca aataaacagg acatggaaca ggccatgact tcctcagaga 481 tggcaaattc acttgggtta cctgccttga aggaccgaaa atggcagata ttcaaaacgt 541 cagcaaccaa aggcaccggc cttgatgagg caatggaatg gttagttgaa acattaaaaa 601 gcagccagta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 661 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 721 tcaacctctg gatta