Construct: ORF ccsbBroad304_05972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010524.1_s304c1, BRDN0000406715
- Derived from:
- ccsbBroadEn_05972
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC25C (995)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05972
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 995 | CDC25C | cell division cycle 25C | NM_001287582.2 | 99.9% | 99.7% | 208C>T |
| 2 | human | 995 | CDC25C | cell division cycle 25C | NM_001790.5 | 99.9% | 99.7% | 208C>T |
| 3 | human | 995 | CDC25C | cell division cycle 25C | NM_001318098.2 | 96.4% | 96.3% | 1_51del;259C>T |
| 4 | human | 995 | CDC25C | cell division cycle 25C | XM_011543759.3 | 95.1% | 94.9% | 1_72del;280C>T |
| 5 | human | 995 | CDC25C | cell division cycle 25C | NM_001364027.1 | 92.7% | 92.6% | 208C>T;759_760ins102 |
| 6 | human | 995 | CDC25C | cell division cycle 25C | NM_001364028.1 | 90.9% | 88.5% | 193_194ins95;240_241ins34 |
| 7 | human | 995 | CDC25C | cell division cycle 25C | XM_006714739.3 | 88.5% | 85.6% | (many diffs) |
| 8 | human | 995 | CDC25C | cell division cycle 25C | NM_001287583.2 | 85.7% | 85.6% | 1_234del;442C>T |
| 9 | human | 995 | CDC25C | cell division cycle 25C | NM_022809.3 | 84.5% | 82.4% | 193_194ins95;238_239ins124 |
| 10 | human | 995 | CDC25C | cell division cycle 25C | XM_011543762.1 | 84.5% | 82.4% | 193_194ins95;238_239ins124 |
| 11 | human | 995 | CDC25C | cell division cycle 25C | NM_001364026.1 | 81.9% | 81.8% | 1_234del;442C>T;1098_1099ins63 |
| 12 | human | 995 | CDC25C | cell division cycle 25C | XM_005272145.3 | 79.6% | 79.4% | 1_234del;442C>T;993_994ins102 |
| 13 | human | 995 | CDC25C | cell division cycle 25C | XM_011543763.1 | 76.5% | 76.5% | 0_1ins333 |
| 14 | human | 995 | CDC25C | cell division cycle 25C | XM_011543760.2 | 75.8% | 75.6% | 1_234del;442C>T;996_997ins165 |
| 15 | human | 995 | CDC25C | cell division cycle 25C | XM_011543761.2 | 72.5% | 70.7% | 1_234del;427_428ins95;472_473ins124 |
| 16 | human | 995 | CDC25C | cell division cycle 25C | XM_011543764.1 | 65.7% | 65.7% | 0_1ins486 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tacggaactc ttctcatcca caagagagga aggaagctct ggctcaggac 121 ccagttttag gtctaatcaa aggaaaatgt taaacctgct cctggagaga gacacttcct 181 ttaccgtctg tccagatgtc cctagaactc cagtgggcaa atttcttggt gattctgcaa 241 acctaagcat tttgtctgga ggaaccccaa aatgttgcct cgatctttcg aatcttagca 301 gtggggagat aactgccact cagcttacca cttctgcaga ccttgatgaa actggtcacc 361 tggattcttc aggacttcag gaagtgcatt tagctgggat gaatcatgac cagcacctaa 421 tgaaatgtag cccagcacag cttctttgta gcactccgaa tggtttggac cgtggccata 481 gaaagagaga tgcaatgtgt agttcatctg caaataaaga aaatgacaat ggaaacttgg 541 tggacagtga aatgaaatat ttgggcagtc ccattactac tgttccaaaa ttggataaaa 601 atccaaacct aggagaagac caggcagaag agatttcaga tgaattaatg gagttttccc 661 tgaaagatca agaagcaaag gtgagcagaa gtggcctata tcgctccccg tcgatgccag 721 agaacttgaa caggccaaga ctgaagcagg tggaaaaatt caaggacaac acaataccag 781 ataaagttaa aaaaaagtat ttttctggcc aaggaaagct caggaagggc ttatgtttaa 841 agaagacagt ctctctgtgt gacattacta tcactcagat gctggaggaa gattctaacc 901 aggggcacct gattggtgat ttttccaagg tatgtgcgct gccaaccgtg tcagggaaac 961 accaagatct gaagtatgtc aacccagaaa cagtggctgc cttactgtcg gggaagttcc 1021 agggtctgat tgagaagttt tatgtcattg attgtcgcta tccatatgag tatctgggag 1081 gacacatcca gggagcctta aacttatata gtcaggaaga actgtttaac ttctttctga 1141 agaagcccat cgtccctttg gacacccaga agagaataat catcgtgttc cactgtgaat 1201 tctcctcaga gaggggcccc cgaatgtgcc gctgtctgcg tgaagaggac aggtctctga 1261 accagtatcc tgcattgtac tacccagagc tatatatcct taaaggcggc tacagagact 1321 tctttccaga atatatggaa ctgtgtgaac cacagagcta ctgccctatg catcatcagg 1381 accacaagac tgagttgctg aggtgtcgaa gccagagcaa agtgcaggaa ggggagcggc 1441 agctgcggga gcagattgcc cttctggtga aggacatgag cccatgccca actttcttgt 1501 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1561 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a