Construct: ORF ccsbBroad304_05999

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002970.1_s304c1, BRDN0000407991
Derived from:
ccsbBroadEn_05999
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRM5 (1133)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05999
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1133 CHRM5 cholinergic receptor muscar... NM_001320917.2 99.8% 99.8% 528G>T;635T>C
2 human 1133 CHRM5 cholinergic receptor muscar... NM_012125.4 99.8% 99.8% 528G>T;635T>C
3 mouse 213788 Chrm5 cholinergic receptor, musca... NM_205783.2 88.1% 88.5% (many diffs)
4 mouse 213788 Chrm5 cholinergic receptor, musca... XM_006499099.3 88.1% 88.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1662
ORF length:
1596
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga aggggattct taccacaatg caaccaccgt caatggcacc ccagtaaatc
121accagccttt ggaacgccac aggttgtggg aagtcatcac cattgcagct gtgactgctg
181tggtaagcct gatcaccatt gtgggcaatg tcttggtcat gatctccttc aaagtcaaca
241gccagctcaa gacagttaac aactattacc tgctcagctt agcctgtgca gatctcatca
301ttggaatctt ctccatgaac ctctacacca cctacatcct catgggacgc tgggctctcg
361ggagtctggc ttgtgacctt tggcttgcac tggactacgt ggccagcaac gcttctgtca
421tgaaccttct ggtgatcagt tttgaccgtt acttttccat cacaagaccc ttgacatatc
481gggccaagcg tactccgaaa agggctggca tcatgattgg cttggcctgg ctgatctcct
541tcatcctctg ggccccagca atcctctgct ggcagtactt ggttgggaag cgtacagttc
601cactggatga gtgccagatc cagtttctct ctgagcccac catcactttt ggcactgcca
661ttgctgcctt ctacatccct gtttctgtca tgaccatccc ctactgtcga atctaccggg
721aaacagagaa gcgaaccaag gacctggctg acctccaggg ttctgactct gtgaccaaag
781ctgagaagag aaagccagct catagggctc tgttcagatc ctgcttgcgc tgtcctcgac
841ccaccctggc ccagcgggaa aggaaccagg cctcctggtc atcctcccgc aggagcacct
901ccaccactgg gaagccatcc caagccactg gcccaagcgc caattgggcc aaagctgagc
961agctcaccac ctgtagcagc tacccttcct cagaggatga ggacaagccc gccactgacc
1021ctgtcctcca agtggtctac aagagtcagg gtaaggaaag cccaggggaa gaattcagtg
1081ctgaagagac tgaggaaact tttgtgaaag ctgaaactga aaaaagtgac tatgacaccc
1141caaactacct tctgtctcca gcagctgctc atagacccaa gagtcagaaa tgtgtggcct
1201ataagttccg attggtggta aaagctgacg ggaaccagga gaccaacaat ggctgtcaca
1261aggtgaaaat catgccctgc cccttcccag tggccaagga accttcaacg aaaggcctca
1321atcccaaccc cagccatcaa atgaccaaac gaaagagagt ggtcctagtc aaagagagga
1381aagcagccca gacactgagt gccattctcc tggccttcat catcacatgg accccgtata
1441acatcatggt cctggtttct accttctgtg acaagtgtgt cccagtcacc ctgtggcact
1501tgggctattg gttgtgctat gtcaatagca ctgtcaaccc catctgctat gccctctgca
1561acagaacctt caggaagacc tttaagatgc tgcttctctg ccgatggaaa aagaaaaaag
1621tggaagagaa gttgtactgg caggggaaca gcaagctacc ctgcccaact ttcttgtaca
1681aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1741ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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