Construct: ORF ccsbBroad304_06076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014694.1_s304c1, BRDN0000461446
- Derived from:
- ccsbBroadEn_06076
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP8B1 (1582)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06076
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1582 | CYP8B1 | cytochrome P450 family 8 su... | NM_004391.3 | 99.8% | 99.6% | 262T>C;1173C>T;1361T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1572
- ORF length:
- 1503
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttctctgg ggtccagtgc tgggagctct gctggtggtc attgctggat 121 acctgtgcct gccagggatg ctccgacaac gcaggccatg ggagccccct ctggacaagg 181 gtaccgtgcc ctggcttggc catgccatgg ctttccggaa gaatatgttt gaatttctga 241 agcgcatgag gaccaagcat ggggatgtgt tcacagtgca gctagggggc cagtacttca 301 ccttcgtcat ggaccccctc tcctttggcc ccatcctcaa ggacacacag agaaaactag 361 actttgggca atatgcaaaa aaactggtgc tgaaggtatt tggataccgt tcagtgcaag 421 gggaccatga gatgatacac tcagccagca ccaagcatct gaggggggat ggcttgaagg 481 atcttaatga gaccatgctg gacagcctgt cctttgtaat gctgacgtcc aaaggctgga 541 gtctggatgc cagttgctgg catgaggaca gcctctttcg cttctgctat tacatcttgt 601 tcacagctgg ctacctgagc ttgttcggct acacgaagga caaggagcag gacctgctac 661 aggcaggaga gttattcatg gagttccgca agtttgacct tcttttccca aggtttgtct 721 actccctgct gtggccccgg gagtggctag aagtgggccg actccagcgt ctctttcaca 781 agatgctctc cgtgagccac agccaggaga aggagggcat cagcaactgg ctgggcaaca 841 tgcttcagtt tctgagggag cagggggtac cctcagctat gcaggacaag ttcaacttca 901 tgatgctctg ggcctcccag gggaacacgg ggcctacctc tttctgggcc ctcttgtacc 961 tcctgaagca cccagaagct attcgggctg tgagggagga agctacccag gtcctgggtg 1021 aggccaggct ggagaccaag cagtcctttg ccttcaaact cggtgccctg caacacaccc 1081 cagttctaga cagcgtggtg gaggagacgc tgcggctgag ggctgcaccc accctcctca 1141 ggttggttca tgaagactat accctgaaga tgtccagtgg gcaggagtat ctgttccgcc 1201 atggagacat cctggccctc tttccctacc tctcagtgca tatggaccct gacatccacc 1261 ctgagcccac cgtcttcaag tacgatcgct tcctcaaccc taatggcagc cggaaagtgg 1321 acttcttcaa gacaggcaag aagatccacc actacaccat gccctggggt tcgggcgttt 1381 ccatctgccc tgggaggttc tttgcactca gtgaggtgaa gctctttacc ctgcttatgg 1441 tcacacactt tgacttagag ttggtggacc ctgacacacc actaccccat gttgacccgc 1501 agcgctgggg ttttggcacc atgcagccca gccacgatgt gcgcttccgc taccgcctgc 1561 atcctacaga gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1621 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1681 caatcaacct ctggatta