Construct: ORF ccsbBroad304_06142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008295.1_s304c1, BRDN0000408347
- Derived from:
- ccsbBroadEn_06142
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF2 (1938)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06142
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1938 | EEF2 | eukaryotic translation elon... | NM_001961.4 | 99.9% | 100% | 1632T>C;2190T>C |
| 2 | mouse | 13629 | Eef2 | eukaryotic translation elon... | NM_007907.2 | 88.3% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2643
- ORF length:
- 2574
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgaacttc acggtagacc agatccgcgc catcatggac aagaaggcca 121 acatccgcaa catgtctgtc atcgcccacg tggaccatgg caagtccacg ctgacagact 181 ccctggtgtg caaggcgggc atcatcgcct cggcccgggc cggggagaca cgcttcactg 241 atacccggaa ggacgagcag gagcgttgca tcaccatcaa gtcaactgcc atctccctct 301 tctacgagct ctcggagaat gacttgaact tcatcaagca gagcaaggac ggtgccggct 361 tcctcatcaa cctcattgac tcccccgggc atgtcgactt ctcctcggag gtgactgctg 421 ccctccgagt caccgatggc gcattggtgg tggtggactg cgtgtcaggc gtgtgcgtgc 481 agacggagac agtgctgcgg caggccattg ccgagcgcat caagcctgtg ctgatgatga 541 acaagatgga ccgcgccctg ctggagctgc agctggagcc cgaggagctc taccagactt 601 tccagcgcat cgtggagaac gtgaacgtca tcatctccac ctacggcgag ggcgagagcg 661 gccccatggg caacatcatg atcgatcctg tcctcggtac cgtgggcttt gggtctggcc 721 tccacgggtg ggccttcacc ctgaagcagt ttgccgagat gtatgtggcc aagttcgccg 781 ccaaggggga gggccagttg gggcctgccg agcgggccaa gaaagtagag gacatgatga 841 agaagctgtg gggtgacagg tactttgacc cagccaacgg caagttcagc aagtcagcca 901 ccagccccga agggaagaag ctgccacgca ccttctgcca gctgatcctg gaccccatct 961 tcaaggtgtt tgatgcgatc atgaatttca agaaagagga gacagcaaaa ctgatagaga 1021 aactggacat caaactggac agcgaggaca aggacaaaga aggcaaaccc ctgctgaagg 1081 ctgtgatgcg ccgctggctg cctgccggag acgccttgtt gcagatgatc accatccacc 1141 tgccctcccc tgtgacggcc cagaagtacc gctgcgagct cctgtacgag gggcccccgg 1201 acgacgaggc tgccatgggc attaaaagct gtgaccccaa aggccctctt atgatgtata 1261 tttccaaaat ggtgccaacc tccgacaaag gtcggttcta cgcctttgga cgagtcttct 1321 cggggctggt ctccactggc ctgaaggtca ggatcatggg gcccaactat acccctggga 1381 agaaggagga cctctacctg aagccaatcc agagaacaat cttgatgatg ggccgctacg 1441 tggagcccat cgaggatgtg ccttgtggga acattgtggg cctcgtgggc gtggaccagt 1501 tcctggtgaa gacgggcacc atcaccacct tcgagcacgc gcacaacatg cgggtgatga 1561 agttcagcgt cagccctgtt gtcagagtgg ccgtggaggc caagaacccg gctgacctgc 1621 ccaagctggt ggaggggctg aagcggctgg ccaagtccga ccccatggtg cagtgcatca 1681 tcgaggagtc gggagagcac atcatcgcgg gcgccggcga gctgcacctg gagatctgcc 1741 tgaaggacct ggaggaggac cacgcctgca tccccatcaa gaaatctgac ccggtcgtct 1801 cgtaccgcga gacggtcagt gaagagtcga acgtgctctg cctctccaag tcccccaaca 1861 agcacaaccg gctgtacatg aaggcgcggc ccttccccga cggcctggcc gaggacatcg 1921 ataaaggcga ggtgtccgcc cgtcaggagc tcaagcagcg ggcgcgctac ctggccgaga 1981 agtacgagtg ggacgtggct gaggcccgca agatctggtg ctttgggccc gacggcaccg 2041 gccccaacat cctcaccgac atcaccaagg gtgtgcagta cctcaacgag atcaaggaca 2101 gtgtggtggc cggcttccag tgggccacca aggagggcgc actgtgtgag gagaacatgc 2161 ggggtgtgcg cttcgacgtc cacgacgtca ccctgcacgc cgacgccatc caccgcggag 2221 ggggccagat catccccaca gcacggcgct gcctctacgc cagtgtgctg accgcccagc 2281 cacgcctcat ggagcccatc taccttgtgg agatccagtg tccagagcag gtggtcggtg 2341 gcatctacgg ggttttgaac aggaagcggg gccacgtgtt cgaggagtcc caggtggccg 2401 gcacccccat gtttgtggtc aaggcctatc tgcccgtcaa cgagtccttt ggcttcaccg 2461 ctgacctgag gtccaacacg ggcggccagg cgttccccca gtgtgtgttt gaccactggc 2521 agatcctgcc cggagacccc ttcgacaaca gcagccgccc cagccaggtg gtggcggaga 2581 cccgcaagcg caagggcctg aaagaaggca tccctgccct ggacaacttc ctggacaaat 2641 tgttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2701 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2761 tctggatta