Construct: ORF ccsbBroad304_06353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017210.1_s304c1, BRDN0000398793
- Derived from:
- ccsbBroadEn_06353
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBB (3043)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06353
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3043 | HBB | hemoglobin subunit beta | NM_000518.5 | 99.5% | 100% | 9T>C;153T>C |
| 2 | human | 3045 | HBD | hemoglobin subunit delta | NM_000519.4 | 92.7% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gcacctgact cctgaggaga agtctgccgt tactgccctg tggggcaagg 121 tgaacgtgga tgaagttggt ggtgaggccc tgggcaggct gctggtggtc tacccttgga 181 cccagaggtt ctttgagtcc tttggggatc tgtccacccc tgatgctgtt atgggcaacc 241 ctaaggtgaa ggctcatggc aagaaagtgc tcggtgcctt tagtgatggc ctggctcacc 301 tggacaacct caagggcacc tttgccacac tgagtgagct gcactgtgac aagctgcacg 361 tggatcctga gaacttcagg ctcctgggca acgtgctggt ctgtgtgctg gcccatcact 421 ttggcaaaga attcacccca ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg 481 ctaatgccct ggcccacaag tatcactacc caactttctt gtacaaagtg gttggtaagc 541 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 601 ggcgcgttaa gtcgacaatc aacctctgga tta