Construct: ORF ccsbBroad304_06553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001785.1_s304c1, BRDN0000399534
- Derived from:
- ccsbBroadEn_06553
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEA8 (4107)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06553
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4107 | MAGEA8 | MAGE family member A8 | NM_001166400.1 | 99.8% | 100% | 954T>G |
| 2 | human | 4107 | MAGEA8 | MAGE family member A8 | NM_001166401.1 | 99.8% | 100% | 954T>G |
| 3 | human | 4107 | MAGEA8 | MAGE family member A8 | NM_005364.5 | 99.8% | 100% | 954T>G |
| 4 | human | 4107 | MAGEA8 | MAGE family member A8 | XM_017029520.1 | 99.8% | 100% | 954T>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tcttgggcag aagagtcagc gctacaaggc tgaggaaggc cttcaggccc 121 aaggagaggc accagggctt atggatgtgc agattcccac agctgaggag cagaaggctg 181 catcctcctc ctctactctg atcatgggaa cccttgagga ggtgactgat tctgggtcac 241 caagtcctcc ccagagtcct gagggtgcct cctcttccct gactgtcacc gacagcactc 301 tgtggagcca atccgatgag ggttccagca gcaatgaaga ggaggggcca agcacctccc 361 cggacccagc tcacctggag tccctgttcc gggaagcact tgatgagaaa gtggctgagt 421 tagttcgttt cctgctccgc aaatatcaaa ttaaggagcc ggtcacaaag gcagaaatgc 481 ttgagagtgt catcaaaaat tacaagaacc actttcctga tatcttcagc aaagcctctg 541 agtgcatgca ggtgatcttt ggcattgatg tgaaggaagt ggaccctgcc ggccactcct 601 acatccttgt cacctgcctg ggcctctcct atgatggcct gctgggtgat gatcagagta 661 cgcccaagac cggcctcctg ataatcgtcc tgggcatgat cttaatggag ggcagccgcg 721 ccccggagga ggcaatctgg gaagcgttga gtgtgatggg gctgtatgat gggagggagc 781 acagtgtcta ttggaagctc aggaagctgc tcacccaaga gtgggtgcag gagaactacc 841 tggagtaccg ccaggcgccc ggcagtgatc ctgtgcgcta cgagttcctg tggggtccaa 901 gggcccttgc tgaaaccagc tatgtgaaag tcctggagca tgtggtcagg gtcaatgcaa 961 gagttcgcat ttcctaccca tccctgcatg aagaggcttt gggagaggag aaaggagtgt 1021 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1081 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1141 ggatta