Construct: ORF ccsbBroad304_06600
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007877.1_s304c1, BRDN0000407315
- Derived from:
- ccsbBroadEn_06600
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT3 (4504)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06600
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4504 | MT3 | metallothionein 3 | NM_005954.4 | 99.5% | 98.5% | 191G>C |
| 2 | mouse | 17751 | Mt3 | metallothionein 3 | NM_013603.2 | 88.2% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 270
- ORF length:
- 204
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ccctgagacc tgcccctgcc cttctggtgg ctcctgcacc tgcgcggact 121 cctgcaagtg cgagggatgc aaatgcacct cctgcaagaa gagctgctgc tcctgctgcc 181 ctgcggagtg tgagaagtgt gccaaggact gtgtgtgcaa aggcggagag gcagctgagg 241 cagaagcaga gaagtccagc tgctgccagt gcccaacttt cttgtacaaa gtggttggta 301 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 361 ggtggcgcgt taagtcgaca atcaacctct ggatta