Construct: ORF ccsbBroad304_06643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009059.1_s304c1, BRDN0000403657
- Derived from:
- ccsbBroadEn_06643
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMB (4828)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06643
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4828 | NMB | neuromedin B | NM_205858.2 | 99.7% | 99.3% | 217C>A |
| 2 | human | 4828 | NMB | neuromedin B | NM_021077.4 | 76.4% | 72% | 217C>A;328_332delCAGTA;363_364ins104 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccggcgggcg gggggcgctc ggatgttcgg cagcctcctg ctcttcgccc 121 tgctcgctgc cggcgtcgcc ccgctcagct gggatctccc ggagccccgc agccgagcca 181 gcaagatccg agtgcactcg cgaggcaacc tctgggccac cggtcacttc atgggcaaga 241 agagtctgga gccttccagc ccatccccat tggggacagc tacccacacc tccctgaggg 301 accagcgact gcagctgagt catgatctgc tcggaatcct cctgctaaag aaggctctgg 361 gcgtgagcct cagccgcccc gcaccccaaa tccaggaggc tgctggtaca aatactgcag 421 aaatgacacc aataatgggg cagacacaac agcgtggctt agattgtgcc cacccaggga 481 aggtgctgaa tgggaccctg ttgatggccc catctggatg taaatcctgc ccaactttct 541 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 601 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta