Construct: ORF ccsbBroad304_06910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013272.1_s304c1, BRDN0000409668
- Derived from:
- ccsbBroadEn_06910
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAA4 (6291)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06910
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6291 | SAA4 | serum amyloid A4, constitutive | NM_006512.4 | 99.7% | 99.2% | 266G>A |
| 2 | human | 100528017 | SAA2-SAA4 | SAA2-SAA4 readthrough | NM_001199744.2 | 62.3% | 62% | 1_234del;500G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gcttttcaca ggcattgttt tctgctcctt ggtcatggga gtcaccagtg 121 aaagctggcg ttcgtttttc aaggaggctc tccaaggggt tggggacatg ggcagagcct 181 attgggacat aatgatatcc aatcaccaaa attcaaacag atatctctat gctcggggaa 241 actatgatgc tgcccaaaga ggacctgggg gtgtctgggc tgctaaactc atcagccgtt 301 ccagggtcta tcttcaggga ttaatagact actatttatt tggaaacagc agcactgtat 361 tggaggactc gaagtccaac gagaaagctg aggaatgggg ccggagtggc aaagaccccg 421 accgcttcag acctgacggc ctgcctaaga aatactgccc aactttcttg tacaaagtgg 481 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 541 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta