Construct: ORF ccsbBroad304_06940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010746.1_s304c1, BRDN0000407968
- Derived from:
- ccsbBroadEn_06940
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFTPD (6441)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06940
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6441 | SFTPD | surfactant protein D | NM_003019.5 | 99.8% | 99.4% | 65A>G;1021G>A |
| 2 | human | 6441 | SFTPD | surfactant protein D | XM_011540087.1 | 99.8% | 99.4% | 65A>G;1021G>A |
| 3 | human | 6441 | SFTPD | surfactant protein D | XM_011540088.2 | 89.4% | 89% | 65A>G;315_316ins117;904G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gctcttcctc ctctctgcac tggtcctgct cacacagccc ctgggctacc 121 tggaagcagg aatgaagacc tactcccaca gaacaatgcc cagtgcttgc accctggtca 181 tgtgtagctc agtggagagt ggcctgcctg gtcgcgatgg acgggatggg agagagggcc 241 ctcggggcga gaagggggac ccaggtttgc caggagctgc agggcaagca gggatgcctg 301 gacaagctgg cccagttggg cccaaagggg acaatggctc tgttggagaa cctggaccaa 361 agggagacac tgggccaagt ggacctccag gacctcccgg tgtgcctggt ccagctggaa 421 gagaaggtcc cctggggaag caggggaaca taggacctca gggcaagcca ggcccaaaag 481 gagaagctgg gcccaaagga gaagtaggtg ccccaggcat gcagggctcg gcaggggcaa 541 gaggcctcgc aggccctaag ggagagcgag gtgtccctgg tgagcgtgga gtccctggaa 601 acacaggggc agcagggtct gctggagcca tgggtcccca gggaagtcca ggtgccaggg 661 gacccccggg attgaagggg gacaaaggca ttcctggaga caaaggagca aagggagaaa 721 gtgggcttcc agatgttgct tctctgaggc agcaggttga ggccttacag ggacaagtac 781 agcacctcca ggctgctttc tctcagtata agaaagttga gctcttccca aatggccaaa 841 gtgtcgggga gaagattttc aagacagcag gctttgtaaa accatttacg gaggcacagc 901 tgctgtgcac acaggctggt ggacagttgg cctctccacg ctctgccgct gagaatgccg 961 ccttgcaaca gctggtcgta gctaagaacg aggctgcttt cctgagcatg actgattcca 1021 agacagaggg caagttcacc taccccacag gagagtccct ggtctattcc aactgggccc 1081 cagggaagcc caacgatgat ggcgggtcag aggactgtgt ggagatcttc accaatggca 1141 agtggaatga cagggcttgt ggagaaaagc gtcttgtggt ctgcgagttc tgcccaactt 1201 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1261 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta