Construct: ORF ccsbBroad304_07059

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004777.1_s304c1, BRDN0000409655
Derived from:
ccsbBroadEn_07059
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGM4 (7047)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07059
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7047 TGM4 transglutaminase 4 NM_003241.4 99.8% 99.7% (many diffs)
2 human 7047 TGM4 transglutaminase 4 XM_011534042.2 93.6% 93.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2118
ORF length:
2052
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat ggatgcatca aaagagctgc aagttctcca cattgacttc ttgaatcagg
121acaacgccgt ttctcaccac acatgggagt tccaaacgag cagtcctgtg ttccggcgag
181gacaggtgtt tcacctgcgg ctggtgctga nccagcccct acaatcctac caccaactga
241aactggaatt cagcacaggg ccgaatccta gcatcgccaa acacaccctg gtggtgctcg
301acccgaggac gccctcagac cactacaact ggcaggcaac ccttcaaaat gagtctggca
361aagaggtcac agtggctgtc accagttccc ccaatgccat cctgggcaag taccaactaa
421acgtgaaaac tggaaaccac atccttaagt ctgaagaaaa catcctatac cttctcttca
481acccatggtg taaagaggac atggttttca tgcctgatga ggacgagcgc aaagagtaca
541tcctcaatga cacgggctgc cattacgtgg gggctgccag aagtatcaaa tgcaaaccct
601ggaactttgg tcagtttgag aaaaatgtcc tggactgctg catttccctg ctgactgaga
661gctccctcaa gcccacagat aggagggacc ccgtgctggt gtgcagggcc atgtgtgcta
721tgatgagctt tgagaaaggc cagggcgtgc tcattgggaa ttggactggg gactacgaag
781gtggcacagc cccatacaag tggacaggca gtgccccgat cctgcagcag tactacaaca
841cgaagcaggc tgtgtgcttt ggccagtgct gggtgtttgc tgggatcctg actacagtgc
901tgagagcgtt gggcatccca gcacgcagtg tgacaggctt cgattcagct cacgacacag
961aaaggaacct cacggtggac acctatgtga atgagaatgg cgagaaaatc accagtatga
1021cccacgactc tgtctggaat ttccatgtgt ggacggatgc ctggatgaag cgaccggatc
1081tgcccaaggg ctacgacggc tggcaggctg tggacgcaac gtcgcaggag cgaagccagg
1141gtgtcttctg ttgtgggcca tcaccactga ccgccatccg caaaggtgac atctttattg
1201tctatgacac cagattcgtc ttctcagaag tgaatggtga caggctcatc tggttggtga
1261agatggtgaa tgggcaggag gagttacacg taatttcaat ggagaccaca agcatcggga
1321aaaacatcag caccaaggca gtgggccaag acaggcggag agatatcacc tatgagtaca
1381agtatccaga aggctcctct gaggagaggc aggtcatgga tcatgccttc ctccttctca
1441gttctgagag ggagcacaga cgacctgtaa aagagaactt tcttcacatg tcggtacaat
1501cagatgatgt gctgctggga aactctgtta atttcaccgt gattcttaaa aggaagaccg
1561ctgccctaca gaatgtcaac atcttgggtt cctttgaact acagttgtac actggcaaga
1621agatggcaaa actgtgtgac ctcaataaga cctcgcagat ccaaggtcaa gtatcagaag
1681tgactctgac cttggactcc aagacctaca tcaacagcct ggctatatta gatgatgagc
1741cagttatcag aggtttcatc attgcggaaa ttgtggagtc taaggaaatc atggcctctg
1801aagtattcac gtctttccag taccctgagt tctctataga gttgcctaac acaggcagaa
1861ttggccagct acttgtctgc aattgtatct tcaagaatac cctggccatc cctttgactg
1921acgtcaagtt ctctttggaa agcctgggca tctcctcact acagacctct gaccatggga
1981cggtgcagcc tggtgagacc atccaatccc aaataaaatg caccccaata aaaactggac
2041ccaagaaatt tatcgtcaag ttaagttcca aacaagtgaa agagattaat gctcagaaga
2101ttgttctcat caccaagtac ccaactttct tgtacaaagt ggttggtaag cctatcccta
2161accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2221agtcgacaat caacctctgg atta

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