Construct: ORF ccsbBroad304_07059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004777.1_s304c1, BRDN0000409655
- Derived from:
- ccsbBroadEn_07059
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGM4 (7047)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07059
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7047 | TGM4 | transglutaminase 4 | NM_003241.4 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 7047 | TGM4 | transglutaminase 4 | XM_011534042.2 | 93.6% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2118
- ORF length:
- 2052
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat ggatgcatca aaagagctgc aagttctcca cattgacttc ttgaatcagg 121 acaacgccgt ttctcaccac acatgggagt tccaaacgag cagtcctgtg ttccggcgag 181 gacaggtgtt tcacctgcgg ctggtgctga nccagcccct acaatcctac caccaactga 241 aactggaatt cagcacaggg ccgaatccta gcatcgccaa acacaccctg gtggtgctcg 301 acccgaggac gccctcagac cactacaact ggcaggcaac ccttcaaaat gagtctggca 361 aagaggtcac agtggctgtc accagttccc ccaatgccat cctgggcaag taccaactaa 421 acgtgaaaac tggaaaccac atccttaagt ctgaagaaaa catcctatac cttctcttca 481 acccatggtg taaagaggac atggttttca tgcctgatga ggacgagcgc aaagagtaca 541 tcctcaatga cacgggctgc cattacgtgg gggctgccag aagtatcaaa tgcaaaccct 601 ggaactttgg tcagtttgag aaaaatgtcc tggactgctg catttccctg ctgactgaga 661 gctccctcaa gcccacagat aggagggacc ccgtgctggt gtgcagggcc atgtgtgcta 721 tgatgagctt tgagaaaggc cagggcgtgc tcattgggaa ttggactggg gactacgaag 781 gtggcacagc cccatacaag tggacaggca gtgccccgat cctgcagcag tactacaaca 841 cgaagcaggc tgtgtgcttt ggccagtgct gggtgtttgc tgggatcctg actacagtgc 901 tgagagcgtt gggcatccca gcacgcagtg tgacaggctt cgattcagct cacgacacag 961 aaaggaacct cacggtggac acctatgtga atgagaatgg cgagaaaatc accagtatga 1021 cccacgactc tgtctggaat ttccatgtgt ggacggatgc ctggatgaag cgaccggatc 1081 tgcccaaggg ctacgacggc tggcaggctg tggacgcaac gtcgcaggag cgaagccagg 1141 gtgtcttctg ttgtgggcca tcaccactga ccgccatccg caaaggtgac atctttattg 1201 tctatgacac cagattcgtc ttctcagaag tgaatggtga caggctcatc tggttggtga 1261 agatggtgaa tgggcaggag gagttacacg taatttcaat ggagaccaca agcatcggga 1321 aaaacatcag caccaaggca gtgggccaag acaggcggag agatatcacc tatgagtaca 1381 agtatccaga aggctcctct gaggagaggc aggtcatgga tcatgccttc ctccttctca 1441 gttctgagag ggagcacaga cgacctgtaa aagagaactt tcttcacatg tcggtacaat 1501 cagatgatgt gctgctggga aactctgtta atttcaccgt gattcttaaa aggaagaccg 1561 ctgccctaca gaatgtcaac atcttgggtt cctttgaact acagttgtac actggcaaga 1621 agatggcaaa actgtgtgac ctcaataaga cctcgcagat ccaaggtcaa gtatcagaag 1681 tgactctgac cttggactcc aagacctaca tcaacagcct ggctatatta gatgatgagc 1741 cagttatcag aggtttcatc attgcggaaa ttgtggagtc taaggaaatc atggcctctg 1801 aagtattcac gtctttccag taccctgagt tctctataga gttgcctaac acaggcagaa 1861 ttggccagct acttgtctgc aattgtatct tcaagaatac cctggccatc cctttgactg 1921 acgtcaagtt ctctttggaa agcctgggca tctcctcact acagacctct gaccatggga 1981 cggtgcagcc tggtgagacc atccaatccc aaataaaatg caccccaata aaaactggac 2041 ccaagaaatt tatcgtcaag ttaagttcca aacaagtgaa agagattaat gctcagaaga 2101 ttgttctcat caccaagtac ccaactttct tgtacaaagt ggttggtaag cctatcccta 2161 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2221 agtcgacaat caacctctgg atta