Construct: ORF ccsbBroad304_07217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012865.1_s304c1, BRDN0000462178
- Derived from:
- ccsbBroadEn_07217
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AXIN2 (8313)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07217
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8313 | AXIN2 | axin 2 | NM_004655.4 | 99.8% | 99.8% | 148C>T;1365A>G;1386C>T |
| 2 | human | 8313 | AXIN2 | axin 2 | XM_011525319.2 | 99.8% | 99.8% | 148C>T;1365A>G;1386C>T |
| 3 | human | 8313 | AXIN2 | axin 2 | XM_011525320.1 | 99.8% | 99.8% | 148C>T;1365A>G;1386C>T |
| 4 | human | 8313 | AXIN2 | axin 2 | XM_011525321.2 | 99.8% | 99.8% | 148C>T;1365A>G;1386C>T |
| 5 | human | 8313 | AXIN2 | axin 2 | XM_017025192.1 | 99.8% | 99.8% | 148C>T;1365A>G;1386C>T |
| 6 | human | 8313 | AXIN2 | axin 2 | NM_001363813.1 | 92.1% | 92.1% | (many diffs) |
| 7 | human | 8313 | AXIN2 | axin 2 | XM_017025193.1 | 92.1% | 92.1% | (many diffs) |
| 8 | mouse | 12006 | Axin2 | axin 2 | NM_015732.4 | 85.4% | 89.8% | (many diffs) |
| 9 | mouse | 12006 | Axin2 | axin 2 | XM_006532057.3 | 85.4% | 89.8% | (many diffs) |
| 10 | mouse | 12006 | Axin2 | axin 2 | XM_006532058.1 | 85.4% | 89.8% | (many diffs) |
| 11 | mouse | 12006 | Axin2 | axin 2 | XM_006532059.3 | 85.4% | 89.8% | (many diffs) |
| 12 | mouse | 12006 | Axin2 | axin 2 | XM_006532060.1 | 85.4% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2598
- ORF length:
- 2529
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtagcgct atgttggtga cttgcctccc ggaccccagc agcagcttcc 121 gtgaggatgc cccgcggccc ccagtgccag gggaagaagg ggagacccca ccgtgtcagc 181 caggggtggg caagggccag gtcaccaaac ccatgtctgt ctcttccaac accaggcgga 241 acgaagatgg gttgggggag ccggaggggc gggcatctcc ggattcccct ctgacccggt 301 ggaccaagtc cttacactcc ttattgggcg atcaagacgg tgcttacctg ttccgaactt 361 tcctggagag ggagaaatgc gtggatacct tagacttctg gtttgcctgc aatggattca 421 ggcagatgaa cctgaaggat accaaaactt tacgagtagc caaagcgatc tacaaaaggt 481 acattgagaa caacagcatt gtctccaagc agctgaagcc tgccaccaag acctacataa 541 gagatggcat caagaagcag cagattgatt ccatcatgtt tgaccaggcg cagaccgaga 601 tccagtcggt gatggaggaa aatgcctacc agatgttttt gacttctgat atatacctcg 661 aatatgtgag gagtggggga gaaaacacag cttacatgag taatggggga ctcgggagcc 721 taaaggtcgt gtgtggctat ctccccacct tgaatgaaga agaggagtgg acttgtgccg 781 acttcaagtg caaactttcg ccaaccgtgg ttggcttgtc cagcaaaact ctgagggcca 841 cggcgagtgt gaggtccacg gaaactgttg acagtggata caggtccttc aagaggagcg 901 atcctgttaa tccttatcac ataggttctg gctatgtctt tgcaccagcc accagcgcca 961 acgacagtga gatatccagt gatgcgctga cggatgattc catgtccatg acggacagca 1021 gtgtagatgg aattcctcct tatcgtgtgg gcagtaagaa acagctccag agagaaatgc 1081 atcgcagtgt gaaggccaat ggccaagtgt ctctacctca tttcccgaga acccaccgcc 1141 tgcccaagga gatgaccccc gtggaacccg ccacctttgc agctgagctg atctcgaggc 1201 tggaaaagct gaagctggag ttggagagcc gccacagcct ggaggagcgc ctgcagcaga 1261 tccgagagga tgaagagaga gagggctccg agctcacact caattcgcgg gagggggcgc 1321 ccacgcagca ccccctctcc ctactgccct ccggcagcta cgaggaagac ccgcagacga 1381 tactggacga tcacctgtcc agggtcctca agacccctgg ctgccagtct ccgggcgtag 1441 gccgctatag ccctcgctcc cgctccccgg accaccacca ccaccaccat tcgcagtacc 1501 actccctgct cccgcccggt ggcaagctgc ctcccgcggc cgcctcgccg ggcgcctgcc 1561 ccctcctcgg gggcaaaggc tttgtgacca agcagacgac gaagcatgtc caccaccact 1621 acatccacca ccatgccgtc cccaagacca aggaggagat cgaggcggag gccacgcagc 1681 gggtgcactg cttctgccct gggggcagcg agtattactg ctactcgaaa tgcaaaagcc 1741 actccaaggc tccggaaacc atgcccagcg agcagtttgg cggcagcaga ggcagtacct 1801 tgcccaaacg caatgggaaa ggcacggagc cgggcctggc cctgcccgcc agggaaggag 1861 gggcccccgg cggagctggg gccctgcagc ttccccggga ggaaggagac aggtcgcagg 1921 atgtctggca gtggatgctg gagagtgagc ggcagagcaa gcccaagccc catagtgccc 1981 aaagcacaaa aaaggcctac cccttggagt ctgcccgctc gtctccaggc gaacgagcca 2041 gccggcacca tctgtggggg ggcaacagcg ggcacccccg caCCACCCCC CGTGCCCACC 2101 TGTTCACCCA GGACCCTGCG ATGCCTCCCC TGACCCCACC CAACACGCTG GCTCAGCTGG 2161 AGGAGGCCTG TCGCAGGCTA GCTGAGGTGT CGAAGCCCCC AAAGCAGCGG TGCTGTGTGG 2221 CCAGTCAGCA GAGGGACAGG AATCATTCGG CCACTGTTCA GACGGGAGCC ACACCCTTCT 2281 CCAATCCAAG CCTGGCTCCA GAAGATCACA AAGAGCCAAA GAAACTGGCA GGTGTCCACG 2341 CGCTCCAGGC CAGTGAGTTG GTTGTCACTT ACTTTTTCTG TGGGGAAGAA ATTCCATACC 2401 GGAGGATGCT GAAGGCTCAG AGCTTGACCC TGGGCCACTT TAAAGAGCAG CTCAGCAAAA 2461 AGGGAAATTA TAGGTATTAC TTCAAAAAAG CAAGCGATGA GTTTGCCTGT GGAGCGGTGT 2521 TTGAGGAGAT CTGGGAGGAT GAGACGGTGC TCCCGATGTA TGAAGGCCGG ATTCTGGGCA 2581 AAGTGGAGCG GATCGATTTG CCAACTTTCT TGTACaaagt ggttggtaag cctatcccta 2641 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2701 agtcgacaat caacctctgg atta