Construct: ORF ccsbBroad304_07254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013688.1_s304c1, BRDN0000400406
- Derived from:
- ccsbBroadEn_07254
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CST7 (8530)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07254
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8530 | CST7 | cystatin F | NM_003650.4 | 86.6% | 86.8% | 0_1ins66;270G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gcctgagaag gcactgcacg gccaccccca actgccccgc actgtcccta 121 cccgggcagc catgcgagcg gctggaactc tgctggcctt ctgctgcctg gtcttgagca 181 ccactggggg cccttcccca gatacttgtt cccaggacct taactcacgt gtgaagccag 241 gatttcctaa aacaataaag accaatgacc caggagtcct ccaagcagcc agatacagtg 301 ttgaaaagtt caacaactgc acgaacgaca tgttcttgtt caaggagtcc cgcatcacaa 361 gggccctagt tcagatagtg aaaggcctga aatatatgct cgaggtggaa attggcagaa 421 ctacctgcaa gaaaaaccag cacctgcgtc tggatgactg tgacttccaa accaaccaca 481 ccttgaagca gactctgagc tgctactctg aagtctgggt cgtgccctgg ctccagcact 541 tcgaggtgcc tgttctccgt tgtcactgcc caactttctt gtacaaagtg gttggtaagc 601 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 661 ggcgcgttaa gtcgacaatc aacctctgga tta