Construct: ORF ccsbBroad304_07455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004116.1_s304c1, BRDN0000409097
- Derived from:
- ccsbBroadEn_07455
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISG15 (9636)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07455
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9636 | ISG15 | ISG15 ubiquitin like modifier | NM_005101.4 | 99.5% | 99.3% | 248G>A;294A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgggacctg acggtgaaga tgctggcggg caacgaattc caggtgtccc 121 tgagcagctc catgtcggtg tcagagctga aggcgcagat cacccagaag atcggcgtgc 181 acgccttcca gcagcgtctg gctgtccacc cgagcggtgt ggcgctgcag gacagggtcc 241 cccttgccag ccagggcctg ggccccggca gcacggtcct gctggtggtg gacaaatgcg 301 acgaacctct gaacatcctg gtgaggaata acaagggccg cagcagcacc tacgaggtgc 361 ggctgacgca gaccgtggcc cacctgaagc agcaagtgag cgggctggag ggtgtgcagg 421 acgacctgtt ctggctgacc ttcgagggga agcccctgga ggaccagctc ccgctggggg 481 agtacggcct caagcccctg agcaccgtgt tcatgaatct gcgcctgcgg ggaggcggca 541 cagagcctgg cgggcggagc tacccaactt tcttgtacaa agtggttggt aagcctatcc 601 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 661 ttaagtcgac aatcaacctc tggatta