Construct: ORF ccsbBroad304_07552
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002885.1_s304c1, BRDN0000461153
- Derived from:
- ccsbBroadEn_07552
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSFM (10102)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07552
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10102 | TSFM | Ts translation elongation f... | NM_005726.6 | 99.8% | 99.6% | 587A>C |
| 2 | human | 10102 | TSFM | Ts translation elongation f... | NM_001172696.2 | 93.8% | 93.6% | 484_546del;650A>C |
| 3 | human | 10102 | TSFM | Ts translation elongation f... | NM_001172697.1 | 62.5% | 57.7% | (many diffs) |
| 4 | human | 10102 | TSFM | Ts translation elongation f... | NM_001172695.2 | 51.2% | 49.5% | 483_484ins88;499A>C;501_502ins386 |
| 5 | mouse | 66399 | Tsfm | Ts translation elongation f... | NM_025537.3 | 84.4% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1044
- ORF length:
- 975
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgctgctg cggtcgctgc gcgtgtttct ggtcgcgcgg accgggagct 121 acccggctgg gtctcttctg cgtcagtcgc cccagccaag gcacacattt tatgctgggc 181 cccgtctgtc tgcctcggcc tccagcaagg agctcctcat gaagctgcgg cggaaaacag 241 gctactcctt tgtaaattgc aagaaagctc tggagacttg tggcggggac ctcaaacagg 301 cagagatctg gctccacaag gaggcccaga aggagggctg gagcaaagct gccaagctcc 361 aagggaggaa gaccaaagaa ggcctgattg ggctgttgca ggaaggaaac acaactgtat 421 tagtagaggt aaactgtgag acagattttg tttctagaaa tttaaaattt caactgttgg 481 tccagcaagt agcccttgga accatgatgc attgtcagac cctaaaggat caaccctctg 541 catacagtaa aggtttcttg aattcctctg agctttctgg acttccagct gggcctgaca 601 gagaaggctc actcaaggat cagttggctt tagcaattgg aaaactggga gaaaccatga 661 ttcttaaacg agctgcatgg gtgaaggtgc catctgggtt ctacgttggc tcttatgtcc 721 acggagcaat gcagagtccc tcacttcaca agctggtgct ggggaagtat ggggccctgg 781 tcatctgtga gacgtctgaa cagaaaacaa accttgaaga cgttggccgc cgccttgggc 841 agcatgtggt gggcatggcc cccctctctg ttggctccct ggacgatgag cctgggggag 901 aggcagagac taagatgctg tcccagccgt atttgctgga tccctccatt accttggggc 961 agtatgtgca gcctcagggg gtgtcggtag tagactttgt gcggtttgaa tgtggagaag 1021 gtgaagaggc agcagaaact gaattgccaa ctttcttgta caaagtggtt ggtaagccta 1081 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1141 gcgttaagtc gacaatcaac ctctggatta