Construct: ORF ccsbBroad304_07617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003648.1_s304c1, BRDN0000405005
- Derived from:
- ccsbBroadEn_07617
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN4 (10473)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07617
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10473 | HMGN4 | high mobility group nucleos... | NM_006353.3 | 99.6% | 100% | 198G>A |
| 2 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010172.2 | 99.6% | 100% | 198G>A |
| 3 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010173.2 | 99.6% | 100% | 198G>A |
| 4 | human | 3151 | HMGN2 | high mobility group nucleos... | NM_005517.4 | 85.9% | 86.6% | (many diffs) |
| 5 | mouse | 15331 | Hmgn2 | high mobility group nucleos... | NM_016957.3 | 82.9% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 336
- ORF length:
- 270
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc caagagaaag gcaaaaggag atgctaaagg tgataaagca aaggtgaagg 121 atgagccaca gaggagatca gctcggttgt ctgctaaacc agctcctcca aaaccagagc 181 ccaggcctaa aaaggcctct gcaaagaagg gagagaagct tcccaaaggg agaaagggga 241 aagcagatgc tggaaaggat ggaaacaacc ctgcaaaaaa ccgagatgcc tctacactcc 301 agtcccagaa agcggaaggc actggggatg ccaagtgccc aactttcttg tacaaagtgg 361 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 421 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta