Construct: ORF ccsbBroad304_07915

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009117.1_s304c1, BRDN0000405589
Derived from:
ccsbBroadEn_07915
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MKRN2 (23609)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07915
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23609 MKRN2 makorin ring finger protein 2 NM_014160.5 99.8% 99.7% 636T>C;1244A>T
2 human 23609 MKRN2 makorin ring finger protein 2 NM_001271707.1 89.5% 89.4% 21_22ins129;507T>C;1115A>T
3 mouse 67027 Mkrn2 makorin, ring finger protei... NM_023290.2 84.8% 88.2% (many diffs)
4 mouse 67027 Mkrn2 makorin, ring finger protei... XM_011241431.1 45.3% 45.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1314
ORF length:
1248
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag caccaagcag atcacttgca ggtattttat gcatggtgtg tgtcgggaag
121gaagtcagtg cctattctca catgacttgg caaacagcaa accgtccacc atctgcaagt
181actaccagaa gggctactgt gcctatggaa ctcggtgcag atatgaccac acgaggccct
241ctgctgcagc tggaggtgct gtgggcacca tggcccacag tgtgccctcc ccagctttcc
301acagtcctca ccctccttcc gaggtcactg catccattgt gaaaactaac tcacatgaac
361ccggaaagcg tgaaaagaga acattggttc ttagagaccg aaatctctct ggcatggctg
421aaaggaagac ccagccgagc atggtgagta atccaggcag ctgcagcgac ccccagccca
481gccccgagat gaagccgcat tcctacctgg atgccatcag gagtggcctt gatgacgtgg
541aggccagcag ctcctacagc aacgagcagc agctgtgccc ctacgcagct gctggggagt
601gccggtttgg ggatgcctgt gtctacctgc acggggaggt gtgtgaaatc tgtaggctgc
661aagtcttgca cccattcgac ccagagcaga ggaaggctca cgaaaagatc tgcatgttga
721cgttcgaaca cgagatggaa aaggcctttg ccttccaggc aagccaggac aaagtgtgca
781gtatctgcat ggaagtgatc ctggagaagg cctctgcttc tgagaggaga tttgggattc
841tctccaattg caatcacacg tactgtttgt cctgcatccg gcagtggcgg tgtgccaaac
901agtttgaaaa cccaatcatt aagtcttgtc cagaatgccg tgtgatatca gagtttgtaa
961ttccaagtgt gtattgggtg gaagatcaga ataaaaagaa cgagttgatt gaagctttca
1021aacaggggat ggggaaaaaa gcctgtaaat actttgagca aggcaagggg acctgcccat
1081ttggaagcaa atgtctttat cgccatgctt accccgatgg gcggctagca gagcctgaga
1141aacctcggaa acagctcagt tctcaaggca ctgtgaggtt ctttaattca gtgcggctct
1201gggatttcat cgagaaccga gaaagccggc atgtccccaa caatgaagat gtcgacatga
1261cagagctcgg ggacctcttc atgcaccttt ctggagtgga atcatcagta ccctacccaa
1321ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1381cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)