Construct: ORF ccsbBroad304_07997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003023.1_s304c1, BRDN0000411118
- Derived from:
- ccsbBroadEn_07997
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTG2 (26164)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07997
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26164 | MTG2 | mitochondrial ribosome asso... | NM_015666.3 | 99.9% | 100% | 21C>T |
| 2 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_005260393.1 | 99.9% | 100% | 21C>T |
| 3 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_011528776.1 | 99.9% | 100% | 21C>T |
| 4 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_017027806.1 | 99.9% | 100% | 21C>T |
| 5 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_006723774.3 | 96.1% | 96.2% | 1_48del;69C>T |
| 6 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_024451869.1 | 96.1% | 96.2% | 1_48del;69C>T |
| 7 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_017027805.1 | 93.4% | 93.5% | 1_84del;105C>T |
| 8 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_017027808.1 | 77.8% | 67.2% | 21C>T;825_850del;996_997ins248 |
| 9 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_017027807.1 | 72.9% | 63% | (many diffs) |
| 10 | human | 26164 | MTG2 | mitochondrial ribosome asso... | XM_024451870.1 | 43.8% | 43.8% | 0_1ins684 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc acctgcaagg tgtttttcag caagattgag gaccgtgttt cagggcgtgg 121 ggcattgggc tttgtccaca tgggctggcc tgaagcccag ccggctactg ccacagcggg 181 cttctcccag gctgctctcg gtcggccgtg cggacctcgc caagcatcag gaactcccgg 241 ggaagaagct gctctctgag aaaaagctga aaaggtactt tgtggactat cggagagtgc 301 ttgtctgtgg aggaaacgga ggcgctgggg caagctgctt ccacagtgag ccccgcaagg 361 agtttggagg ccctgatgga ggggacggag gcaacggtgg acacgtcatt ctgagagttg 421 accagcaagt caagtccctg tcgtcggtcc tgtcgcggta ccagggtttc agtggagaag 481 atggagggag taaaaactgc ttcgggcgca gtggcgccgt cctctacatc cgggtccccg 541 tgggcacgct ggtgaaggag ggaggcagag ttgtggccga cctgtcttgc gtgggagatg 601 agtacattgc cgcgctgggc ggggcaggag ggaaaggcaa ccgcttcttc ctggccaaca 661 acaaccgtgc ccctgtgacc tgtacccctg gacagccagg acagcagcga gttctccacc 721 tggagctcaa gacggtggcc cacgccggaa tggtgggatt ccccaacgcc gggaagtcct 781 cactgctccg ggccatttca aacgccagac ccgccgtggc ttcctacccg ttcaccaccc 841 tgaagcccca cgtcgggatc gtccactacg aaggccacct acaaatagca gtggccgaca 901 tccccggcat catacgaggc gcccaccaga acaggggtct ggggtccgcc ttcctcaggc 961 acatcgagcg ctgccgcttt ctcttgttcg tggtggatct ttctcagcct gagccgtgga 1021 ctcaagttga cgatttaaaa tatgaactgg agatgtatga aaagggcctg tctgcgaggc 1081 cccacgcaat cgtcgcaaac aagattgacc tccctgaagc ccaagccaat ctgtcccagc 1141 tccgggatca cttgggacag gaggtcatcg tgctgtcggc gttgaccggc gagaacctgg 1201 agcagctgct gttgcacctg aaggtgctgt atgacgccta cgcggaggcc gagctgggcc 1261 agggccgcca gccgctcagg tggtacccaa ctttcttgta caaagtggtt ggtaagccta 1321 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1381 gcgttaagtc gacaatcaac ctctggatta