Construct: ORF ccsbBroad304_08231

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002777.1_s304c1, BRDN0000410654
Derived from:
ccsbBroadEn_08231
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHCHD2 (51142)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08231
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51142 CHCHD2 coiled-coil-helix-coiled-co... NM_016139.4 99.7% 99.3% 161G>T
2 human 51142 CHCHD2 coiled-coil-helix-coiled-co... NM_001320327.1 89.6% 88% 161G>T;445_448delGGTA;458_504del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
519
ORF length:
453
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gcgtggaagc cgaagccgca cctcccgcat ggcccctccg gccagccggg
121cccctcagat gagagctgca cccaggccag caccagtcgc tcagccacca gcagcggcac
181ccccatctgc agttggctct tctgctgctg cgccccggca gccagttctg atggcccaga
241tggcaaccac tgcagctggc gtggctgtgg gctctgctgt ggggcacaca ttgggtcacg
301ccattactgg gggcttcagt ggaggaagta atgctgagcc tgcgaggcct gacatcactt
361accaggagcc tcagggaacc cagccagcac agcagcagca gccttgcctc tatgagatca
421aacagtttct ggagtgtgcc cagaaccagg gtgacatcaa gctctgtgag ggtttcaatg
481aggtgctgaa acagtgccga cttgcaaacg gattggccta cccaactttc ttgtacaaag
541tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
601gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)