Construct: ORF ccsbBroad304_08545

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002319.1_s304c1, BRDN0000461498
Derived from:
ccsbBroadEn_08545
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CNOT11 (55571)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08545
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55571 CNOT11 CCR4-NOT transcription comp... NM_017546.5 99.8% 99.8% 162G>T;1519A>G
2 mouse 52846 Cnot11 CCR4-NOT transcription comp... NM_028043.2 89.5% 95.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1596
ORF length:
1530
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc cggcggaggg gcgagcgcgg cgtctggccg gcttctcacc gccgcggagc
121aaagagggtc ccgggaagcg gcagggtcgg cgtccaggag cggcttcggg ggctccggcg
181gcggcagagg cggagcaagc ggccccgggt ccgggagcgg aggccctggg ggccccgcgg
241gcaggatgag cttgaccccg aaggagctct cgagcctgct gagcatcata tcggaggagg
301cgggcggcgg cagcaccttc gagggcctgt ccaccgcctt ccaccactac ttcagcaagg
361ccgaccactt ccgcctgggc tcggtgctcg tcatgctgct ccagcagccc gacctgctgc
421ctagcgcggc gcagcgcctc acggcgctct acctgctctg ggagatgtac cgcaccgagc
481cgctggccgc caaccccttc gccgccagct tcgcgcacct gctcaacccc gcgccgcccg
541cccgcggcgg ccaggaaccc gaccgccctc cgctctcagg atttttacct cctataactc
601caccagaaaa gttttttctt tcccagctga tgctggcacc cccacgggaa ctcttcaaaa
661agacgcctcg ccagattgca ctgatggacg ttggaaacat gggccagtct gtggacatta
721gtgggcttca gttagccttg gccgaacgcc aatctgaatt gccaacgcaa agcaaagcga
781gcttccccag tattctcagt gacccagacc cggattcttc taattctgga tttgacagct
841cagttgcctc tcagatcaca gaagctttag tcagcggacc aaagccacct attgaaagcc
901attttcgacc agagtttatt cgtccaccgc ctccactcca catttgtgag gatgaacttg
961cttggctaaa ccccacggag cctgaccacg cgatccagtg ggataaatcg atgtgtgtta
1021agaatagcac tggtgtggag atcaaacgaa taatggccaa agccttcaaa agccccttat
1081cctctcccca acaaacacag ctacttggtg agttggaaaa agaccccaaa cttgtctacc
1141atattggcct caccccagcc aaacttcctg accttgtgga aaacaaccct ttagtcgcta
1201tagaaatgtt gctgaaatta atgcagtcaa gccagatcac tgagtatttc tctgtcctgg
1261tcaatatgga catgtcttta cattcaatgg aagttgtaaa tcgactaact acagctgttg
1321atctacctcc tgaatttatt cacctttata tatcaaattg catctctact tgtgaacaga
1381ttaaggataa atatatgcag aatcggttgg tgcgtcttgt gtgtgtgttt ctccaatcct
1441tgatccgtaa caaaattatt aatgtacagg atttgtttat agaagtgcag gcattctgta
1501ttgaattcag taggatacga gaagctgctg gtcttttccg gttgttgaag acattggata
1561ctggggaaac accttctgag accgaaatgt caaaataccc aactttcttg tacaaagtgg
1621ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1681ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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