Construct: ORF ccsbBroad304_08632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000700.1_s304c1, BRDN0000402007
- Derived from:
- ccsbBroadEn_08632
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHA3 (56145)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08632
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56145 | PCDHA3 | protocadherin alpha 3 | NM_031497.1 | 99.9% | 99.8% | 866T>C |
| 2 | human | 56145 | PCDHA3 | protocadherin alpha 3 | NM_018906.3 | 84.4% | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2541
- ORF length:
- 2472
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgttctcc tggcgagaag atcctggagc ccagtgcctg ctgctttctc 121 ttctgctcct cgcagcctcg gaggtgggga gcggccagct ccactactcc gtctctgagg 181 aggccaagca tggcaccttc gtgggccgca tcgcgcagga cctggggctg gagctggcgg 241 agctggtgcc gcgcctgttc cgggtggcgt ccaaaagaca cggggacctt ctggaggtaa 301 atctgcagaa tggcattttg tttgtgaatt ctcggataga ccgcgaggaa ctgtgcgggc 361 ggagcgcgga gtgcagcatc cacctggagg tgatcgtgga caggccgctg caggttttcc 421 atgtggaggt ggaagtgaag gacattaatg acaacgcgcc agtttttcca atggctgtaa 481 agaatctgtt tatttccgaa tcccgacagc ctggctctcg gttttcgcta gagggcgcat 541 cagatgcaga tatcggaaca aattcgttgt tgacttacag tcttgattcc actgaatatt 601 ttaccttgga cgttaaaaga aatgatgagg aaattaaatc ccttggactc gtgttgaaaa 661 aaaatttaaa tcgagaggac actcctaagc attatttact aataacagca attgatggtg 721 ggaaaccaga gctcactggc acgactcaac taaagatcac tgttttagat gtaaacgaca 781 acgccccagc gtttgagagg acgatctata aagtcagatt actcgaaaat gcaccaaatg 841 gtaccctagt ggtgaccgtt aacgccaccg atttggatga aggagtaaat aaggatatcg 901 cgtattcttt caatacggac atgtcagcag atactctgtc aaaattccat ttagatccag 961 tcaatggaca aatcagtgta aagggtaaca tagatttcga ggaaagtaag tcatatgaaa 1021 tccaggtaga agccacggat aaaggaaatc ccccaatgtc agatcactgc acagttctac 1081 tcgaaattgt ggacatcaat gataatgtac ctgagttagt tattcaatca ctatctttac 1141 ctgtattaga agactctcca cttagcacag tcatcgctct gatcagcgtg tccgaccgcg 1201 actcaggagt caatggacag gtcacctgct cgctgacgcc ccacgtcccc ttcaagctgg 1261 tgtccacctt caagaattac tactcattgg tgctggacag ccctctggac cgcgagagcg 1321 tgtcggccta tgagctggtg gtgactgctc gggacggggg ctcgccttca ctgtgggcca 1381 cggccagcgt gtccgtggag gtggccgacg tgaacgacaa tgcgccggca ttctcgcagt 1441 ccgagtacac ggtgttcgtg aaggagaaca acccgccggg ctgccacatc ttcacggtgt 1501 ctgcgcggga cgcggacgcg caggagaacg ccctggtgtc ctactcgctg gtggaacggc 1561 gggtggggga gcgcgcgctg tcgagctacg tgtcggtaca cgcggagagc ggcaaggtgt 1621 acgcgctgca gccgctggac cacgaggagc tagagctgct gcagttccag gtgagtgcgc 1681 gcgatgcggg cgtgccgcct ctgggcagca acgtgacgct gcaggtgttc gtgctggacg 1741 agaacgacaa cgcgccggca ctgctgatgc ctcgggtggg tggcatcggt ggcgcagtga 1801 gcgagctggt gccgcggtca gtgggtgcgg gccacgtggt agcgaaggtg cgcgcagtgg 1861 atgcagactc aggctacaac gcgtggcttt cgtatgagct gcagcctggg accggcggtg 1921 cgcgcatccc gtttcgcgtg gggctgtaca cgggagagat cagcacgacc cgtgccctgg 1981 acgaggtgga cgccccgcgc catcgcctac tggtgctggt gaaggaccac ggtgaaccct 2041 cattgaccgc cacggccact gtgctggtgt cgctggtgga gagtggccag gcacccaagg 2101 cctcgtccca ggcgtccgct ggcgccacgg gcccggaagc tgcactggtg gatgtcaacg 2161 tgtacttgat cgtcgccatc tgcgcggtgt ccagtctgtt ggtgctcaca ctgctgctat 2221 atactgctct gcggtgctcc gcgccgccaa ccgaaggcga ctgtgggccg ggcaagccca 2281 cgctggtgtg ctccagcgcg gtggggagct ggtcatactc gcagcagagg cagcagaggg 2341 tgtgctctgg agaggggttg cccaagaccg acctcatggc ttttagccct agccttcctc 2401 cttgtccaat tagccgggat agagaggaga aacaggatgt ggacgttgat ctctcagcca 2461 aagtgagtaa tttttattta ttctttccaa aatgtctttg tttttcattc ctcaatgttt 2521 ccactcctct ggaaatacat ttgccaactt tcttgtacaa agtggttggt aagcctatcc 2581 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2641 ttaagtcgac aatcaacctc tggatta