Construct: ORF ccsbBroad304_09025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015628.1_s304c1, BRDN0000401894
- Derived from:
- ccsbBroadEn_09025
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPHKAP (80309)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09025
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | NM_001142644.2 | 99.9% | 99.9% | 755C>T |
| 2 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | XM_005246870.4 | 98.9% | 98.9% | 0_1ins51;704C>T |
| 3 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | XM_011511925.2 | 98.9% | 98.7% | (many diffs) |
| 4 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | XM_006712777.3 | 98.3% | 98.1% | 0_1insATGG;27_105del;830C>T |
| 5 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | NM_030623.3 | 98.2% | 98.2% | 755C>T;4634_4635ins87 |
| 6 | human | 80309 | SPHKAP | SPHK1 interactor, AKAP doma... | XM_006712778.4 | 96.6% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5169
- ORF length:
- 5100
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatggcaac tccctgctct cggtaccaag caacttggag tcatcacgga 121 tgtatgacgt tttggaaccg cagcagggca gaggctgtgg cagctcagga agcggcccgg 181 ggaactccat cacagcctgt aagaaggttc ttcgcagcaa tagcctgctg gagtcaacag 241 actactggtt gcagaatcag aggatgccct gccaaattgg ttttgtagaa gacaagtctg 301 aaaactgtgc ttctgtctgc tttgtgaatc ttgatgtgaa caaggatgaa tgcagcacag 361 agcacctgca acagaaactg gtcaacgttt caccagatct tccaaaactt atcagttcca 421 tgaatgtcca acaaccaaaa gaaaatgaaa ttgttgtcct aagtgggtta gcctctggaa 481 atctccaggc agattttgaa gtttcacagt gcccttggct gccagatatc tgcttggtcc 541 aatgtgcaag agggaacaga ccaaacagta ccaactgcat catctttgaa atcaacaaat 601 ttctgattgg tctggaactg gtgcaggagc gacagctcca cctggaaaca aacatcttga 661 aactggagga tgacacgaac tgttccttat cttcaatcga ggaagacttt ctcaccgctt 721 ctgagcactt ggaggaggaa agcgaggtgg atgaatctag gaacgattat gaaaatataa 781 atgtctcagc caatgttttg gaaagtaaac agctaaaggg agtcacccag gtggaatgga 841 attgcaacaa ggaaaagtgg ctttatgctt tggaagacaa atacatcaac aaatatccca 901 caccattgat taaaacagaa cgatctccag aaaacctaac aaagaacaca gccttgcaga 961 gtctagatcc ctcagccaag ccatcacagt ggaaaagaga agctgtgggg aatgggagac 1021 aagccacaca ttattatcat tcagaagctt ttaaaggtca aatggaaaaa tcacaggcac 1081 tgtatattcc aaaagatgct tatttctcca tgatggataa agatgtacct tctgcatgtg 1141 ctgtggcaga gcagagaagc aacctaaacc caggagacca tgaagacaca agaaacgctc 1201 tccctcctag acaagatgga gaagtcacca ctggcaagta tgctacaaat ttagcagaat 1261 ccgtgctgca ggatgcattt attagattat ctcaatctca gtccacatta ccccaggaat 1321 ctgcagtcag tgtttctgta ggaagttctc tgcttcccag ttgctattcc acaaaagata 1381 cagtggtttc tcggtcatgg aatgagctcc ccaaaatcgt cgttgttcag agtccagatg 1441 gcagtgatgc tgccccacag ccaggcatct cctcctggcc tgagatggaa gtctctgttg 1501 aaacctcaag catcctctct ggagagaact ccagcagaca accccagagt gctctagaag 1561 tggcgttagc ttgtgcagcc actgtgattg gaactatttc cagtccacag gccacagaaa 1621 gactcaaaat ggagcaagtg gtctcgaact ttcccccagg gagcagtggt gcactgcaaa 1681 ctcaagcacc ccaaggactc aaggaacctt ccatcaatga gtactccttt ccatctgctt 1741 tgtgtggcat gactcaggtg gccagtgccg tggctgtctg tggtctgggt gaaagagaag 1801 aggtgacatg ctcagtggct ccaagtggta gcctcccgcc tgcagctgag gcttctgaag 1861 ccatgccccc actttgtggt ttagcaagca tggagcttgg caaggaagcc attgccaagg 1921 gattgctcaa ggaggctgct ctggttttaa caaggcctaa tacctacagc agcattggag 1981 actttctgga ctccatgaac aggagaatca tggaaactgc ttcaaagtct cagaccctgt 2041 gctcagaaaa tgtcgtcagg aatgaactgg cacataccct gtccaatgtt atcctgaggc 2101 attccattga tgaagttcac cacaaaaata tgataatcga ccccaatgac aacaggcatt 2161 catctgaaat tctggacacc ttaatggaaa gtacaaatca actgctttta gatgtgatat 2221 gcttcacgtt caagaagatg agtcatattg tacggcttgg tgaatgtcct gctgtccttt 2281 ctaaggagac catcagaagg agggagacag aaccaagctg ccagccatct gatccgggtg 2341 ctagtcaagc ttggacaaaa gccactgaat cctccagcag ctctccactt agcaattcac 2401 acaacacgag tcttgtcatc aacaatcttg tggatggcat gtattcaaaa caagacaagg 2461 gtggagtgag gccaggcctc ttcaagaacc ccacgctgca gtcacaatta tcacgtagtc 2521 acagagtgcc cgattcttca actgctacaa catcctccaa ggaaatatat ctgaaaggaa 2581 tagcaggaga ggatacaaaa agccctcatc acagtgagaa tgaatgcaga gcctcttccg 2641 aaggacaaag gtccccaacg gtcagccagt ccagaagtgg ttcccaggag gctgaggaga 2701 gtatccaccc aaacacccaa gaaaagtaca actgtgccac atctcgcatc aacgaagttc 2761 aagtcaacct gtccttgtta ggggatgacc tgctgcttcc tgctcaatcc acgcttcaaa 2821 caaagcatcc agacatctac tgcattacag actttgcgga agaattagca gacacggtcg 2881 tctccatggc aactgaaatt gcagcgattt gccttgacaa ctccagtgga aaacaaccct 2941 ggttttgtgc atggaaaaga gggagtgagt ttctgatgac acccaacgta ccctgccgat 3001 ccttgaagag gaagaaagag agccagggga gcgggaccgc tgtgaggaaa cacaagcctc 3061 cccggctcag tgagatcaag aggaagacgg acgagcaccc tgagcttaaa gaaaagctga 3121 tgaacagggt tgtggatgag tccatgaacc ttgaagatgt cccagattct gtcaatcttt 3181 ttgccaatga agtggcagcc aagatcatga acctaacgga gttctctatg gtggacggca 3241 tgtggcaggc gcagggctat ccccggaatc ggttactgag tggcgacagg tggagccggc 3301 tgaaggcctc cagctgcgaa agcattcctg aggaagactc cgaggccagg gcctatgtca 3361 acagcctggg cttaatgagc acgctgagcc agccggtcag cagggccagc tctgtctcca 3421 agcagtcgag ctgtgagagc atcaccgatg agttttccag gttcatggtg aaccagatgg 3481 aaaatgaagg gagaggattt gagttactgc tggattacta tgctggcaag aacgccagca 3541 gcattctgaa ctcagccatg caacaggcgt gccggaaaag tgaccacctc agtgtgaggc 3601 ctagctgtcc ctctaagcag tccagcacag agagcatcac tgaggagttc tacaggtaca 3661 tgctgaggga catcgaaaga gacagcagag aaagtgcctc ctccagacgg agcagccagg 3721 attggacagc cggcctgctg tctccttctc tgcgatcccc agtgtgccac agacagtcgt 3781 ccatgccaga cagcagatcc ccatgctcca ggctgacagt gaatgtgccc atcaaagcca 3841 actctttaga tggctttgct cagaactgcc cacaagattt cctaagcgtg cagccggtca 3901 gtagcgcgtc ctcatccggt ctctgcaaat ctgactcttg cttgtatcgg agaggtggga 3961 ctgaccacat caccaacatg ttaattcatg aaacgtgggc tagctccatt gaggctctca 4021 tgcgcaagaa caaaatcatt gtggatgatg cagaggaagc tgacactgag cctgtttctg 4081 gtggctctcc ctcgcaagca gagaagtgtg caaatagatt agctgcgagc aggatgtgca 4141 gtgggccaac tctgcttgtt caggagtctc tcgattgccc gaggaaagac tctgttaccg 4201 aatgtaaaca gcccccagtg tcatctttga gcaaaactgc ttctcttaca aaccacagcc 4261 ctttagattc taaaaaagaa acttcctcgt gccaggaccc tgtaccaata aaccacaaaa 4321 ggcgatcact ttgctcgagg gaagtgcctt tgattcagat tgaaacagat cagagagaag 4381 cctgtgctgg ggaacctgaa cccttccttt ccaaaagcag cctcctagag gaagcagaag 4441 ggcattcgaa tgacaaaaac atcccagatg tggtgagagg tggagacaca gccgtgagcg 4501 cttgtcaaat ccatagtgac agccttgata ccagagatgt accagaggct gaagcctcca 4561 cagaagccag agcccccgat gaggccccca accctccaag cagcagcgag gagagcacag 4621 gcagctggac ccagcttgcc aatgaggaag acaacccaga tgacacaagt agctttctcc 4681 agctcagtga gcgatccatg agcaatggca acagtagtgc cactagcagt cttggcatta 4741 tggatctgga catttatcag gaaagcatgc catcttctcc catgattaat gaattagtag 4801 aagaaaagaa gattcttaaa ggacagtcag aaagcacaga ggcacctgca tctggaccgc 4861 ctacgggaac agccagcccc cagaggagcc tgctggtgat caactttgac ctggagccag 4921 agtgtccaga tgccgagctc cgagccactc tgcagtggat agctgcctct gaactgggga 4981 ttcccaccat ctactttaag aaatctcagg aaaacagaat tgaaaagttt ctagatgtcg 5041 tgcagctggt tcatcggaag tcctggaaag tgggtgatat cttccatgca gttgtccagt 5101 actgcaaaat gcatgaggag cagaaggatg ggagactgag tctctttgac tggctcttgg 5161 aactgggatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 5221 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 5281 tcaacctctg gatta