Construct: ORF ccsbBroad304_09150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001980.1_s304c1, BRDN0000407557
- Derived from:
- ccsbBroadEn_09150
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRAM1 (84106)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09150
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84106 | PRAM1 | PML-RARA regulated adaptor ... | NM_032152.5 | 99.9% | 99.8% | 32G>A;1236G>C |
| 2 | human | 84106 | PRAM1 | PML-RARA regulated adaptor ... | XM_011528353.2 | 98.8% | 98% | (many diffs) |
| 3 | human | 84106 | PRAM1 | PML-RARA regulated adaptor ... | XM_005272502.2 | 94.6% | 94.6% | 32G>A;1236G>C;1975_2085del |
| 4 | human | 84106 | PRAM1 | PML-RARA regulated adaptor ... | XM_011528352.2 | 93.6% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2076
- ORF length:
- 2010
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccatcacctg cctgcagcca tggagaacca tcaggacttc cggagcatca 121 aagcaaagtt ccaggcctct cagccggagc ccagcgacct gcccaaaaaa cctccgaagc 181 ctgagtttgg taaactgaag aagttctccc agcctgagct aagcgagcac cccaagaagg 241 ccccgctgcc tgagtttggt gcagtgtcct tgaagccccc gccgcctgag gtcactgacc 301 tccccaagaa gcccccgccg cctgaggtca ctgacctccc caagaagccc ccgccgcctg 361 aggtcactga cctccccaag aagcccccgc cgcctgaggt cactgacctc cccaagaagc 421 cgtccaaact ggagttgagt gacctctcca agaagttccc acagctgggg gccactccgt 481 ttccaaggaa gcccctgcag cctgaggtcg gtgaggcccc tttgaaggcc tcgctgccgg 541 agcctggtgc gccggcccgg aaacccctgc agcccgacga actcagtcac cccgccagac 601 ccccctccga acccaaatcc ggcgcattcc ccaggaagct ctggcaaccc gaggccggtg 661 aggctacccc gaggtccccg cagcctgagt tgagtacctt tcccaagaag cctgcgcagc 721 ctgagttcaa cgtgtacccc aaaaagcctc cgcagcctca ggtcggtggc ctccctaaga 781 agtccgtgcc gcagcctgag ttcagcgagg ccgctcagac tcccctctgg aagcctcagt 841 ccagcgagcc gaagcgcgac tccagcgcct ttcccaaaaa ggcctcccag cctccgctga 901 gtgactttcc caagaagcct ccgcagcctg agcttgggga cctcaccagg acctcctcag 961 agcccgaagt cagcgtgctt cccaagaggc cgcggccggc cgaattcaaa gcgctctcca 1021 agaagccccc gcagcccgag ctgggcggcc tccccaggac ctcctcagag cccgagttca 1081 actcactccc caggaagctg ctgcagccgg agcgccgggg gccaccccgc aagttctcac 1141 agcctgagcc cagcgctgtc ctcaagagac acccgcagcc tgagttcttc ggtgatctcc 1201 ctcgaaagcc tccactcccc agctccgctt ccgagagctc actgcctgcg gccgtggccg 1261 gcttcagctc ccggcacccg ctcagccctg ggtttggagc cgctgggaca ccccgctgga 1321 ggtcaggagg cctggttcac agtggagggg ccaggccagg cctcagaccc agccatccac 1381 cccggcggag gcctctgccc cctgccagca gcctgggaca ccctccagcc aagcccccgc 1441 tgcccccggg gcccgtggat atgcagagct ttcggagacc ctctgcagca tccatagatc 1501 tacggaggac ccgctcggcc gctgggctcc acttccagga ccgacagcct gaagacatcc 1561 cgcaggtccc agatgagatc tacgagctgt atgacgatgt ggaacccaga gatgactcca 1621 gccccagccc caagggcaga gatgaagcgc cctcagttca gcaagccgcc aggaggccac 1681 cacaagaccc agcgctcagg aaggagaagg atccccagcc acagcagttg ccacccatgg 1741 acccaaagtt gctgaagcag ctgaggaagg cagagaaggc cgagagggag ttccggaaga 1801 agttcaagtt tgaaggggag atcgtggttc acacgaagat gatgatcgac cccaacgcta 1861 agacacgtcg cgggggtggc aagcacctcg ggatccggcg cggggagatc ctggaggtga 1921 tcgagttcac cagcaatgag gagatgctgt gccgggaccc caaaggcaaa tatggctacg 1981 tgcccagaac agcgctcctg cccctggaga cggaggtgta cgatgatgtc gacttctgcg 2041 atcccctgga aaaccaacca ctccccctgg gacggttccc aactttcttg tacaaagtgg 2101 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2161 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta