Construct: ORF ccsbBroad304_09316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003695.1_s304c1, BRDN0000405469
- Derived from:
- ccsbBroadEn_09316
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISX (91464)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09316
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91464 | ISX | intestine specific homeobox | NM_001303508.2 | 99.5% | 99.1% | 82A>G;99T>C;169C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 804
- ORF length:
- 735
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtgctgag gtgggccctg ctctctgcag gggtatggag agaaatagct 121 tggggtgctg tgaggccccg aagaagctgg gcctgtcctt ctccatcgag gcgatcctaa 181 agaggcctgc caggaggagt gatatggaca gaccagaagg gccaggtgaa gagggctccg 241 gagaagctgc ggcctcaggc tctgggctag aaaagcctcc aaaggaccag ccccaggaag 301 gaaggaagag caagcggagg gttcgtacca ccttcaccac tgagcagctg catgagctgg 361 agaagatctt ccactttacc cactacccag acgttcacat ccgcagccag ctggcagcca 421 ggatcaacct cccagaagct cgggtgcaga tctggttcca gaatcagcga gccaagtggc 481 ggaagcagga gaagattggc aacctggggg ctccacagca gctgagtgaa gccagtgtgg 541 ccctgcccac aaatctggat gtggctgggc ccacgtggac atccactgct ctgcgcaggc 601 tggctcctcc cacgagctgt tgtccatcgg ctcaagatca gctggcctct gcctggttcc 661 ctgcctggat caccctcctc ccagcgcacc catgggaaac acagcctgtc ccaggtcttc 721 ccatccatca aacttgcatc cctgtgctat gcatccttcc acctccacac cccaaatggg 781 gcagcatctg tgctacttca acattgccaa ctttcttgta caaagtggtt ggtaagccta 841 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 901 gcgttaagtc gacaatcaac ctctggatta