Construct: ORF ccsbBroad304_09629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007313.1_s304c1, BRDN0000408101
- Derived from:
- ccsbBroadEn_09629
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- C16orf89 (146556)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09629
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146556 | C16orf89 | chromosome 16 open reading ... | NM_001098514.2 | 90.2% | 89.9% | (many diffs) |
| 2 | human | 146556 | C16orf89 | chromosome 16 open reading ... | XM_005255143.3 | 79.5% | 78.8% | (many diffs) |
| 3 | human | 146556 | C16orf89 | chromosome 16 open reading ... | NM_152459.4 | 78.4% | 73.4% | (many diffs) |
| 4 | human | 146556 | C16orf89 | chromosome 16 open reading ... | XM_017022972.1 | 71.5% | 69% | (many diffs) |
| 5 | human | 146556 | C16orf89 | chromosome 16 open reading ... | XM_017022973.1 | 71.5% | 69% | (many diffs) |
| 6 | human | 146556 | C16orf89 | chromosome 16 open reading ... | XM_017022974.1 | 71.5% | 69% | (many diffs) |
| 7 | human | 146556 | C16orf89 | chromosome 16 open reading ... | XM_011522392.2 | 36.5% | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tcttcatgtc tgccaggggg aggtgggaag gaggtgggag gagggcgtgc agaggcagtc 121 tgggcttggc cagagctcag ggtgctgagc gtgtgaccag cagtgagcag aggccggcca 181 tggccagcct ggggctgctg ctcctgctct tactgacagc actgccaccg ctgtggtcct 241 cctcactgcc tgggctggac actgctgaaa gtaaagccac cattgcagac ctgatcctgt 301 ctgcgctgga gagagccacc gtcttcctag aacagaggct gcctgaaatc aacctggatg 361 gcatggtggg ggtccgagtg ctggaagagc agctaaaaag tgtccgggag aagtgggccc 421 aggagcccct gctgcagccg ctgagcctgc gcgtggggat gctgggggag aagctggagg 481 ctgccatcca gagatccctc cactacctca agctgagtga tcccaagtac ctaagagagt 541 tccagctgac cctccagccc gggttttgga agctcccaca tgcctggatc cacactgatg 601 cctccttggt gtaccccacg ttcgggcccc aggactcatt ctcagaggag agaagtgacg 661 tgtgcctggt gcagctgctg ggaaccggga cggacagcag cgagccctgc ggcctctcag 721 acctctgcag gagcctcatg accaagcccg gctgctcagg ctactgcctg tcccaccaac 781 tgctcttctt cctctgggcc agaatgaggg gatgcacaca ggcaccactc caacagagcc 841 aggactatat caacctcttc tgcgccaaca tgatggactt gaaccgcaga gctgaggcca 901 tcggatacgc ctaccctacc cgggacatct tcatggaaaa catcatgttc tgtggaatgg 961 gcggcttctc cgacttctac aagctccggt ggctggaggc cattctcagc tggcagaaac 1021 agcaggaagg atgcttcggg gagcctgatg ctgaagatga agaatcatct aaagctattc 1081 aatatcagca gcatttttcg aggagagtga agaggcgaga aaaacaattt ccagatggct 1141 gctcctccca caacacagcc acagcagtgg cagccctggg tggcttccta tacatcctgg 1201 cagaataccc cccagcaaac agagagccac acccatccac accgccacca ccaagcagcc 1261 gctgaccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1321 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1381 tctggatta