Construct: ORF ccsbBroad304_09859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005739.1_s304c1, BRDN0000460374
- Derived from:
- ccsbBroadEn_09859
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLEU7 (220107)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09859
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | NM_198989.3 | 99.7% | 99.3% | 248C>T |
| 2 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | NM_001306135.1 | 71.3% | 69.6% | (many diffs) |
| 3 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | XR_001749491.2 | 10.9% | (many diffs) | |
| 4 | mouse | 239133 | Dleu7 | deleted in lymphocytic leuk... | NM_173419.2 | 57.8% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 549
- ORF length:
- 480
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagccct gcgcccttag tggcctccat cagccaccaa atggtggctc 121 tgcagacctt gcagctgctg cagcaggagt ggggctgggg ggacggtcca gtcgcccccg 181 ggaacccgcg ggacccagac cacgtgtcca ccgctccagc ccgtcgctca ggcccgccgc 241 gggcccggcc ggggcccggg cgcgaggagc ggggcggggg cgtggggacc aggagtcggc 301 ggaccgcggc gcgggtgaac tccccagagg aggaggtagt gcgaggcgct gaggggggcg 361 ccgagttgct gcccttcccc cgggaccgcg ggccctgcac cctggcccag atggcgatgc 421 gcagcgcgct ggcccgcgtg gtggactcga cttcggagct ggtcagcgtg gagcagacgc 481 tgctggggcc cctccagcag gagcggtcct ttcccattca cctgaagctt caactcctgg 541 ctaatgagtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 601 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 661 tcaacctctg gatta