Construct: ORF ccsbBroad304_10060

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003616.1_s304c1, BRDN0000408363
Derived from:
ccsbBroadEn_10060
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GEN1 (348654)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10060
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 348654 GEN1 GEN1 Holliday junction 5' f... NM_001130009.3 99.9% 99.7% 274T>A;929G>A
2 human 348654 GEN1 GEN1 Holliday junction 5' f... NM_182625.5 99.9% 99.7% 274T>A;929G>A
3 human 348654 GEN1 GEN1 Holliday junction 5' f... XM_005262613.4 99.9% 99.7% 274T>A;929G>A
4 human 348654 GEN1 GEN1 Holliday junction 5' f... XM_006712005.3 99.9% 99.7% 274T>A;929G>A
5 human 348654 GEN1 GEN1 Holliday junction 5' f... XM_011532820.2 99.9% 99.7% 274T>A;929G>A
6 human 348654 GEN1 GEN1 Holliday junction 5' f... XM_011532821.2 99.9% 99.7% 274T>A;929G>A
7 human 348654 GEN1 GEN1 Holliday junction 5' f... XM_011532822.2 99.9% 99.7% 274T>A;929G>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2793
ORF length:
2724
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggagtgaat gacttgtggc aaattttgga gcctgttaag caacacatcc
121ccttgcgtaa tcttggtggg aaaaccattg cagttgatct gagtctctgg gtgtgtgagg
181cacagacagt caaaaaaatg atgggcagcg tcatgaagcc ccacctcagg aacttatttt
241ttcgtatctc atatttaaca caaatggatg taaaactggt atttgttatg gaaggggaac
301caccaaagct gaaagctgat gtcataagca agaggaatca gactcggtat gggtcttctg
361gaaaatcgtg gtctcagaaa acagggagat cacattttaa atcagtctta agagagtgcc
421tccatatgct cgaatgctta ggaatcccct gggttcaggc tgctggggaa gctgaagcca
481tgtgtgctta tctcaatgct ggtggtcatg tcgatggctg cctcaccaat gatggagata
541ctttccttta tggggcccag actgtttaca ggaatttcac tatgaataca aaggacccac
601atgttgactg ttacacaatg tcatctatca agagtaaact aggtttggat agagatgctc
661tggttggatt agcaatactt cttggctgtg attatctccc aaagggagtc cctggagttg
721gaaaagagca agcattaaaa cttatacaga ttttgaaagg gcaaagttta cttcagaggt
781ttaatcggtg gaatgaaaca tcttgtaact ctagtccaca actgctagtc actaaaaaac
841tggctcattg ttccgtatgt tcccatccag gttcacctaa ggatcatgaa cgtaatggat
901gcagattatg taaaagtgat aaatattgtg agccacatga ctatgaatac tgctgtcctt
961gtgagtggca ccgtacagaa catgataggc aactcaatga agtagagaac aatattaaga
1021agaaagcttg ctgttgtgag ggattcccat tccatgaggt tattcaagaa ttccttttaa
1081acaaggataa attggtgaag gttatcaggt accaaagacc tgatttgtta ttgtttcaga
1141gatttactct tgaaaaaatg gagtggccca atcactatgc atgtgagaaa ttgctggtac
1201ttttgaccca ttatgacatg atagaaagaa agcttggtag cagaaactct aatcaactac
1261agccaattcg aattgttaag actcgaatca gaaatggagt tcattgtttt gaaatagaat
1321gggaaaagcc tgaacattat gctatggaag ataaacaaca tggagaattt gctttattaa
1381caattgagga agaatcattg tttgaagcag catatcctga gatcgttgct gtttaccaaa
1441aacaaaagtt agaaattaaa gggaagaaac aaaaacgtat taagcctaaa gaaaacaatt
1501tgccagaacc agatgaagta atgagctttc agtcacacat gactttaaaa cccacatgtg
1561aaatctttca taagcagaat tccaagttaa attcggggat ttcccctgat cctacattac
1621cacaggaatc tatttctgcc tcattgaata gcttgctttt acctaaaaat actccatgtt
1681tgaatgcaca agaacagttc atgtcttctc taagaccttt ggctatacag caaattaaag
1741ctgtcagtaa gtctctaatt tcagaatcta gtcaacccaa tacctcatct cataatatat
1801ccgtgattgc tgatctacac ttgagcacta ttgactggga aggtacttct tttagtaatt
1861ctccagctat tcaaaggaat actttttctc atgatttaaa atcagaagtt gaatcagagc
1921tatcagccat ccctgatggc tttgaaaata tcccagaaca actgtcctgt gaatcagaaa
1981ggtacactgc aaacataaag aaagtgttgg atgaggattc tgatgggatt agtcctgaag
2041agcatctact ttctggcatt actgatttat gtcttcagga tttgccttta aaggaacgaa
2101tatttacaaa attatcatat cctcaggata atctacaacc agatgtcaac ctgaaaactt
2161tgtccatact tagtgtaaaa gaatcttgta ttgctaacag tggttctgat tgtacatcac
2221atctttcaaa ggatcttcca ggaattccct tgcaaaatga atccagagac tctaaaattc
2281taaaaggaga ccagctgctt caagaagact ataaagtcaa tacttctgtc ccttattctg
2341tcagtaacac agtggtaaag acctgcaatg ttagaccacc aaatactgct ttagatcata
2401gtagaaaagt tgatatgcaa accactcgga aaattttaat gaagaagagt gtttgccttg
2461acagacattc ctctgatgaa caaagtgccc cagtgtttgg gaaagctaag tacacaactc
2521aaagaatgaa gcacagttct caaaagcata attcatccca tttcaaagaa agtggccata
2581acaagttgag tagccctaag atacatatta aagaaactga acagtgtgtc agatcttatg
2641aaacagctga aaatgaagaa agctgtttcc cagattcaac aaaaagttct ctgagttctc
2701tacaatgtca taagaaagaa aacaactctg gtacttgttt ggatagccct cttcctttac
2761gccagagatt aaaactaaga ttccaaagca ctttgccaac tttcttgtac aaagtggttg
2821gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2881accggtggcg cgttaagtcg acaatcaacc tctggatta

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