Construct: ORF ccsbBroad304_10116

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007098.1_s304c1, BRDN0000409601
Derived from:
ccsbBroadEn_10116
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SHC4 (399694)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10116
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 399694 SHC4 SHC adaptor protein 4 NM_203349.4 99.8% 99.5% 154A>G;730A>G;1340A>G
2 human 399694 SHC4 SHC adaptor protein 4 XM_005254375.3 69.1% 68% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1956
ORF length:
1890
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg agaacgcggc caggacagcc tggcaggact cgtgctgtat gtaggactct
121tcgggcaccc cgggatgctg cacagggcca agtacagccg ctttcggaac gagtcgatca
181cgtccttgga cgaaggtagc tccggaggct cggtcgggga caagggctcg ccgcagcctc
241cccaccccgc cctggcacct cacctgccga ctgaagatgc caccttgccg tcgcaggaga
301gccccacccc actgtgcacc ttgatccccc gcatggcaag catgaagctg gccaacccgg
361ccactttgct gagtctgaaa aacttttgcc tgggtaccaa agaggtgcct cggctgaagc
421tccaggaaag ccgggaccca ggttccagcg gcccctcttc cccagaaacc agtttaagta
481ggtccgggac tgcacctcca ccgcagcagg acctggtggg acacagggca accgccctaa
541cccctgattc gtgcccgctt cctggccctg gggagccaac acttaggagc aggcaggaca
601ggcactttct acagcacctg ttggggatgg gcatgaacta ctgtgtgagg tacatgggct
661gtgttgaagt gctgcaatca atgagatcac tggattttgg aatgagaacc caagttacaa
721gggaagcaat aagtcgcctg tgtgaagctg tccccggggc aaatggagcc attaaaaagc
781gaaagcctcc agttgagttc ctatcaacag tccttggcaa aagtaatctt cagttttcag
841gaatgaatat aaaactgacc atctcaacat gcagtctcac attgatgaat cttgacaacc
901aacagattat tgcaaatcat catatgcagt ctatttcatt tgcctctgga ggggatcctg
961atactacaga ctatgttgcc tacgtagcta aagatccagt taatcaacga gcctgtcaca
1021tattggaatg ccacaatgga atggcccaag acgtcataag taccataggg caggcttttg
1081aactccggtt taaacagtac ttgaaaaatc cttctttgaa tacttcttgt gaaagtgagg
1141aggtgcatat tgatagccat gccgaggaga gagaagatca tgaatattac aatgaaattc
1201cagggaagca gccaccagta ggtggtgttt cagatatgcg gatcaaagtt caagccacgg
1261aacaaatggc ttactgcccc atacagtgtg aaaagttgtg ctatttgcct ggaaactcca
1321agtgcagcag tgtatatgag aactgtttag aacaaagcag ggcaataggt aatgtccatc
1381caagaggggt gcagtcccag cgaggtacct cattattgaa gcacacgtgc cgagtggatc
1441tctttgatga cccctgctac attaatacac aggctcttca aagtacacct ggctctgctg
1501gaaatcaaag gtcagcccaa ccactgggga gcccatggca ctgcggaaag gcaccagaaa
1561ctgttcagcc gggtgccaca gcccagcctg ccagctcaca ttctttgcca cacattaagc
1621agcagctgtg gagcgaagaa tgctatcatg gcaagctgag caggaaggcg gcagagagcc
1681tcttggtaaa ggatggggac tttttggttc gagagagtgc aacatcccct ggccaatatg
1741tgctgagtgg actacaggga ggccaagcaa aacatcttct cctggtggat cctgaaggca
1801aggtgaggac caaggatcat gtatttgata atgtcggcca ccttatcaga taccatatgg
1861ataacagttt gccaatcatc tcctctggaa gcgaagtaag ccttaaacaa ccagtgagaa
1921aagataataa tccagcactt ttgcattcca acaaatgccc aactttcttg tacaaagtgg
1981ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2041ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)