Construct: ORF ccsbBroad304_10148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014323.1_s304c1, BRDN0000403332
- Derived from:
- ccsbBroadEn_10148
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACBD7 (414149)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10148
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414149 | ACBD7 | acyl-CoA binding domain con... | NM_001039844.3 | 99.6% | 100% | 153A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgcaggct gattttgaca gggctgcaga agatgtgagg aagctgaaag 121 caagaccaga tgatggagaa ctgaaagaac tctatgggct ttacaaacaa gcaatagttg 181 gagacattaa tattgcgtgt ccaggaatgc tagatttgaa aggcaaagcc aaatgggaag 241 catggaacct caaaaaaggg ttgtcgacgg aagatgcgac gagtgcctat atttctaaag 301 caaaggagct gatagaaaaa tacggaattt acccaacttt cttgtacaaa gtggttggta 361 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 421 ggtggcgcgt taagtcgaca atcaacctct ggatta