Construct: ORF ccsbBroad304_10272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004921.1_s304c1, BRDN0000402511
- Derived from:
- ccsbBroadEn_10272
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP5-AS1 (84837)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10272
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84837 | ARHGAP5-AS1 | ARHGAP5 antisense RNA 1 (he... | NR_027263.1 | 13.1% | 1_551del;720_1281del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 234
- ORF length:
- 168
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggcccctgat tcagtacgtt ctgtgaaagt ggagcgagaa gccaagacgt 121 ggatcgagaa gccccgtggg gcgggtctca gggtggccca aaagacccct gttcacgcca 181 ctaccagcct aactttagga actgtcgtcc acctggcttt tattatactg ccttacccaa 241 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 301 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta