Construct: ORF ccsbBroad304_10293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011855.1_s304c1, BRDN0000398745
- Derived from:
- ccsbBroadEn_10293
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFL1P1 (142913)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10293
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 142913 | CFL1P1 | cofilin 1 pseudogene 1 | NR_028492.1 | 15.5% | 1_377del;711_2141del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 399
- ORF length:
- 333
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctcccatgtg gctgtctttg atgatgtcat caaggtgttc agtgacatga 121 aagtgtgcaa gtcttcaaca ctagaaaagg tgaagaagcg caagaagttg ctgctcttct 181 gcctgagtga gtacaagaag tacatcatcc ttgcggaggc caagaagatc ctggtaagta 241 atgtggacca aaccattgat gatccctatg ccacttttgt caagatgctg acagtaagga 301 ctgccgctac gccccttatg acgccaccaa ggacagcaag aagaaggacc tggtgtttat 361 cttctgggcc tctgagtctg catccattaa gagcaaaatg cccaactttc ttgtacaaag 421 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 481 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta