Construct: ORF ccsbBroad304_10382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018112.1_s304c1, BRDN0000398665
- Derived from:
- ccsbBroadEn_10382
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB-AS3 (404266)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10382
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404266 | HOXB-AS3 | HOXB cluster antisense RNA 3 | NR_033204.2 | 30.9% | 1_154del;314_514del | |
| 2 | human | 404266 | HOXB-AS3 | HOXB cluster antisense RNA 3 | NR_033201.2 | 26% | 1_251del;411_611del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 225
- ORF length:
- 159
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc agtgctgccg ggaacccagc gatatccgca ccagcggaga aggttccagg 121 ctgccggcgg cggcgcagag agcgggaaga gaggctcgga ggaagccccg ggcgtggcgt 181 ggtcaggctc cgagagcggc cgggatgcgg ccacaccggc ctggtaccca actttcttgt 241 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 301 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a