Construct: ORF ccsbBroad304_10384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003613.1_s304c1, BRDN0000408114
- Derived from:
- ccsbBroadEn_10384
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LOC440173 (440173)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10384
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440173 | LOC440173 | uncharacterized LOC440173 | NR_027471.1 | 11.4% | 1_141del;373_2018del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 297
- ORF length:
- 231
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgagatgaa cccggtacct cagatggaaa tgcagaaatc acccgtcttc tgcgtcgctc 121 acgctgggag ctgtagaccg gagcttattc ggccatcttg gctcctccgt tccctctatg 181 agtccaacca ggatggcgac ttgactggat tttacagttt cattgacacg catgagctct 241 atcaaacttc tgggagtttt tctgaaatgt ttacatggaa ggcacctgga gcccattgaa 301 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 361 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 421 tta