Construct: ORF ccsbBroad304_10394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008684.1_s304c1, BRDN0000401303
- Derived from:
- ccsbBroadEn_10394
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KU-MEL-3 (497048)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10394
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 497048 | KU-MEL-3 | uncharacterized LOC497048 | NR_122107.1 | 14.6% | 1_186del;361_1190del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 243
- ORF length:
- 174
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttccgcga actcctcgcc ccgacctcat ccttctccag ctcctacctg 121 caggcctcag gcctcctgac ctccaggctc cttaccctcc cgctggcctc tgctctacac 181 tcatctttgt gccctgcttc caatcatgcc aggcctcggg gtccctgtct ctaacttcac 241 cattgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 301 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 361 tctggatta