Construct: ORF ccsbBroad304_10397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003792.1_s304c1, BRDN0000407305
- Derived from:
- ccsbBroadEn_10397
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR31HG (554202)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10397
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554202 | MIR31HG | MIR31 host gene | NR_152878.1 | 11.5% | 1_181del;395_1852del | |
| 2 | human | 554202 | MIR31HG | MIR31 host gene | NR_152877.1 | 10.7% | 1_318del;532_1989del | |
| 3 | human | 554202 | MIR31HG | MIR31 host gene | NR_152879.1 | 10% | 1_440del;654_2111del | |
| 4 | human | 554202 | MIR31HG | MIR31 host gene | NR_027054.2 | 9.3% | 1_577del;791_2266del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 279
- ORF length:
- 213
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcctggaaa tacctcctca aggccagtgt agagccctca atcacccact 121 gcccccagct tctccttctc ccatctcctg caccaagcag gtctccaggt gttccagctg 181 ctgatgacgt aaagtgtgga gttggtccag gaaggtcatg ttccatgcag cagaggagcg 241 ctttgtgtga gaagttgaag acctgctgta acacatcata cccaactttc ttgtacaaag 301 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 361 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta