Construct: ORF ccsbBroad304_10533
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006053.1_s304c1, BRDN0000403338
- Derived from:
- ccsbBroadEn_10533
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTBP1-DT (92070)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10533
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92070 | CTBP1-DT | CTBP1 divergent transcript | NR_033339.1 | 9.2% | 1_1316del;1412T>G;1590_2945del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac caccgccctc cagggtcagc gtttggctgt ttgtgtgcct gcccacccgc 121 ctccctgtgc ctgggcctgc cctgtgcctg cacacaggag gggctcatgg cgtttgccct 181 acacggatgg gctgtcctgg gagctactgg acagtcacct tggtgggaat gccagaggca 241 tgggcattag gtccccccgg ccagcctccg ttgccacatg ggctattttt gtccatcgcg 301 tgggacaacc tagtattggg ggaaaactca gtccactcta cccaactttc ttgtacaaag 361 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 421 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta